Department of Biochemistry and Molecular Biology, Farmazia Fakultatea/Facultad de Farmacia, UPV-EHU, Gasteiz, Spain.
Transl Res. 2012 Nov;160(5):332-45. doi: 10.1016/j.trsl.2012.02.012. Epub 2012 Mar 11.
The field of point-of-care (POC) testing technology is developing quickly and producing instruments that are increasingly reliable, while their size is being gradually reduced. Proteins are a common target for POC analyses and the detection of protein markers typically involves immunoassays aimed at detecting different groups of proteins such as tumor markers, inflammation proteins, and cardiac markers; but other techniques can also be used to analyze plasma proteins. In the case of nucleic acids, hybridization and amplification strategies can be used to record electromagnetic or electric signals. These techniques allow for the identification of specific viral or bacterial infections as well as specific cancers. In this review, we consider some of the latest advances in the analysis of specific nucleic acid and protein biomarkers, taking into account their trend toward miniaturization and paying special attention to the technology that can be implemented in future applications, such as lab-on-a-chip instruments.
即时检测（POC）技术领域发展迅速，所生产的仪器越来越可靠，同时其体积也在逐渐缩小。蛋白质是 POC 分析的常见目标，而蛋白质标志物的检测通常涉及免疫分析，旨在检测不同的蛋白质组，如肿瘤标志物、炎症蛋白和心脏标志物；但也可以使用其他技术来分析血浆蛋白。在核酸的情况下，可以使用杂交和扩增策略来记录电磁或电信号。这些技术可以识别特定的病毒或细菌感染以及特定的癌症。在这篇综述中，我们考虑了分析特定核酸和蛋白质生物标志物的一些最新进展，考虑到它们向小型化的趋势，并特别关注可在未来应用中实施的技术，如芯片实验室仪器。
