Lindgren V, Rutberg L
J Bacteriol. 1976 Sep;127(3):1047-57. doi: 10.1128/jb.127.3.1047-1057.1976.
In pleiotropic negative glycerol utilization mutants (GlpPI mutants) of Bacillus subitilis, glycerol kinase and sn-glycerol 3-phosphate (G3P) dehydrogenase are noninducible. GlpPI mutants also fail to take up exogenous [14C]G3P. To study the regulation of the glp system in B. subtilis phenotypically, Glp+ revertants were isolated from GlpPI mutants. Four classes of revertants were identified: phenotypically, wild type; R1 type, which contains an informational suppressor, R2 type, which produced G3P dehydrogenase constitutively; and R3 type, with a temperature-sensitive Glp phenotype producing G3P dehydrogenase constitutively at permissive temperature (32 degrees C). The properties of the revertants indicate that the glpPI locus codes for a protein with a positive regulatory function.
在枯草芽孢杆菌的多效性负甘油利用突变体（GlpPI突变体）中，甘油激酶和sn-甘油-3-磷酸（G3P）脱氢酶是不可诱导的。GlpPI突变体也无法摄取外源[14C]G3P。为了从表型上研究枯草芽孢杆菌中glp系统的调控，从GlpPI突变体中分离出了Glp+回复突变体。鉴定出了四类回复突变体：表型上为野生型；R1型，含有一个信息抑制子；R2型，组成型产生G3P脱氢酶；以及R3型，具有温度敏感的Glp表型，在允许温度（32摄氏度）下组成型产生G3P脱氢酶。回复突变体的特性表明，glpPI位点编码一种具有正调控功能的蛋白质。
