Groupe de recherche en écologie buccale, Faculté de Médecine Dentaire, Pavillon de Médecine dentaire, Université Laval, Québec, Canada.
J Appl Microbiol. 2008 Nov;105(5):1630-9. doi: 10.1111/j.1365-2672.2008.03908.x.
To investigate the efficacy of nisin Z, an antimicrobial peptide produced by certain strains of Lactococcus lactis against Candida albicans growth and transition.
Candida albicans was cultured in the presence of various concentrations of nisin Z (1000, 500, and 100 microg ml(-1)) for different time points. Candida albicans growth was determined using the Alamar Blue assay. The yeast's transition from blastospore to hyphal form was assessed through optical microscope observations. The effect of nisin Z on C. albicans ultrastructure was followed by scanning and transmission electron microscopy. Our results show that nisin Z inhibited C. albicans growth beginning at 500 microg ml(-1). This inhibition was both time- and dose-dependent. Nisin Z was also active against C. albicans transition by significantly inhibiting the transformation of C. albicans from the blastospore to hyphal form. Treatments with nisin Z lead to ultrastructural disturbances of C. albicans.
Our findings indicate that nisin Z significantly reduced C. albicans growth and transition. These effects may have occurred through ultrastructural modifications of this yeast.
For the first time, effect of nisin Z on C. albicans was investigated. These results therefore suggest that nisin Z may have antifungal properties, and could be used as an antifungal molecule.
研究乳链菌肽 Z（某些乳球菌菌株产生的一种抗菌肽）对白色念珠菌生长和形态转变的抑制效果。
在不同时间点，用不同浓度（1000、500 和 100μg/ml）的乳链菌肽 Z 培养白色念珠菌。通过 Alamar Blue 检测法来确定白色念珠菌的生长情况。通过光学显微镜观察评估酵母从芽生孢子到菌丝体的转变。通过扫描和透射电子显微镜观察乳链菌肽 Z 对白色念珠菌超微结构的影响。结果表明，乳链菌肽 Z 从 500μg/ml 开始抑制白色念珠菌生长，这种抑制作用具有时间和剂量依赖性。乳链菌肽 Z 还能显著抑制白色念珠菌从芽生孢子向菌丝体的转变，从而抑制白色念珠菌的转变。乳链菌肽 Z 处理会导致白色念珠菌的超微结构紊乱。
本研究结果表明，乳链菌肽 Z 能显著减少白色念珠菌的生长和形态转变。这些作用可能是通过对该酵母的超微结构进行修饰而产生的。
首次研究了乳链菌肽 Z 对白色念珠菌的作用。因此，这些结果表明，乳链菌肽 Z 可能具有抗真菌特性，并可用作抗真菌分子。
