Huzzey J M, Duffield T F, LeBlanc S J, Veira D M, Weary D M, von Keyserlingk M A G
Faculty of Land and Food Systems, University of British Columbia, Vancouver, British Columbia, Canada.
J Dairy Sci. 2009 Feb;92(2):621-5. doi: 10.3168/jds.2008-1526.
The purpose of this study was to determine whether haptoglobin (Hp) could be used as a predictive measure for metritis. Cattle were grouped into 3 health categories based on the condition of vaginal discharge and body temperature after calving: severe metritis (n = 12), mild metritis (n = 32), and healthy (n = 23). Blood was collected and analyzed for Hp concentration on d -20 +/- 5, -6 +/- 2, -2 +/- 1, and d 0 relative to calving, and then every 3 d after calving until d +21. Cows with mild and severe metritis had greater Hp concentrations than healthy cows between d 0 and d +12. Mean (+/-SE) Hp concentrations peaked on d +3 in the cows with mild metritis (1.06 +/- 0.15 g/L) and on d +6 in cows with severe metritis (1.62 +/- 0.47 g/L). Mean concentrations for the healthy group were 0.58 +/- 0.12 g/L and 0.31 +/- 0.08 g/L on d +3 and d +6, respectively. Clinical signs of pathological discharge for the mildly and severely metritic cows did not occur until, on average, 8.6 +/- 3.9 d and 5.3 +/- 1.9 d after calving, respectively. Cows with Hp concentrations >or=1 g/L on d +3 were 6.7 times more likely to develop severe or mild metritis; this predictive threshold has a sensitivity of 50% and specificity of 87%. These results indicate that an acute phase inflammatory response precedes clinical metritis and that Hp screening may assist in the early detection of metritis, providing increased opportunities for early treatment and prevention.
本研究的目的是确定触珠蛋白（Hp）是否可作为子宫炎的预测指标。根据产犊后阴道分泌物状况和体温，将奶牛分为3个健康类别：严重子宫炎（n = 12）、轻度子宫炎（n = 32）和健康（n = 23）。在相对于产犊的第-20±5天、-6±2天、-2±1天和第0天采集血液并分析Hp浓度，然后在产犊后每隔3天采集一次，直至第+21天。在第0天至第+12天期间，患轻度和严重子宫炎的奶牛的Hp浓度高于健康奶牛。轻度子宫炎奶牛的平均（±标准误）Hp浓度在第+3天达到峰值（1.06±0.15 g/L），严重子宫炎奶牛在第+6天达到峰值（1.62±0.47 g/L）。健康组在第+3天和第+6天的平均浓度分别为0.58±0.12 g/L和0.31±0.08 g/L。轻度和重度子宫炎奶牛出现病理性分泌物的临床症状平均分别在产犊后8.6±3.9天和5.3±1.9天。在第+3天Hp浓度≥1 g/L的奶牛发生严重或轻度子宫炎的可能性高6.7倍；该预测阈值的敏感性为50%，特异性为87%。这些结果表明，急性期炎症反应先于临床子宫炎出现，并且Hp筛查可能有助于子宫炎的早期检测，为早期治疗和预防提供更多机会。
