用于测定血浆中脂质过氧化物的简单且特异的检测方法。
Simple and specific test for measuring lipid peroxides in plasma.
作者信息
Görög P, Kotak D C, Kovacs I B
机构信息
Thrombosis Unit, St Bartholomew's Hospital, West Smithfield, London.
出版信息
J Clin Pathol. 1991 Sep;44(9):765-7. doi: 10.1136/jcp.44.9.765.
The specificity of an iodometric assay for measuring lipid peroxides in lipoproteins was tested, compared with the fluorimetric thiobarbituric acid assay, and adopted for detecting lipid peroxide in plasma samples. Oxidation of low density lipoproteins in vitro by Cu2+, lipoxidase, and phagocytosing polymorphonuclear leucocytes was sensitively detected by the iodometric assay. Unlike the thiobarbituric acid assay, neither non-lipid substances commonly present in plasma, nor platelet or polymorphonuclear leucocyte by-products interfered with the iodometric assay. The iodometric assay measured a normal mean (SD) plasma lipid peroxide concentration of 10.8 (2.1) microM; n = 63. Two weeks after the start of a high cholesterol diet in rabbits (n = 5), a sixfold increase in plasma lipid peroxide concentrations was measured by iodometric assay. The specificity of a simple and sensitive iodometric test of lipid peroxidation was superior to that of the thiobarbituric acid assay. This iodometric assay should therefore provide a much more accurate assessment of lipid peroxide in plasma samples.
对用于测量脂蛋白中脂质过氧化物的碘量法的特异性进行了测试，与荧光硫代巴比妥酸法进行了比较，并采用该方法检测血浆样品中的脂质过氧化物。通过碘量法可灵敏地检测到体外铜离子、脂氧化酶和吞噬性多形核白细胞对低密度脂蛋白的氧化作用。与硫代巴比妥酸法不同，血浆中常见的非脂质物质以及血小板或多形核白细胞的副产物均不会干扰碘量法。碘量法测得正常血浆脂质过氧化物平均浓度（标准差）为10.8（2.1）微摩尔；n = 63。在给兔子（n = 5）开始高胆固醇饮食两周后，通过碘量法测得血浆脂质过氧化物浓度增加了六倍。脂质过氧化作用的一种简单灵敏的碘量测试法的特异性优于硫代巴比妥酸法。因此，这种碘量法应该能更准确地评估血浆样品中的脂质过氧化物。
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