el-Saadani M, Esterbauer H, el-Sayed M, Goher M, Nassar A Y, Jürgens G
Institute of Medical Biochemistry, Graz, Austria.
J Lipid Res. 1989 Apr;30(4):627-30.
A method is described for measuring lipid peroxides by means of the color reagent of a commercially available test kit for cholesterol estimation. In principle, this assay makes use of the oxidative capacity of lipid peroxides to convert iodide to iodine, which can be measured photometrically at 365 nm. Calibration curves were obtained using peroxides such as H2O2, t-butyl hydroperoxide, and cumene hydroperoxide. A stoichiometric relationship was observed between the amount of organic peroxides assayed and the concentration of iodine produced. Concentrations of lipid peroxides as small as 1 nmol/ml could be measured. The ability to estimate lipid peroxides of isolated low density lipoprotein was demonstrated.
描述了一种通过用于胆固醇测定的市售测试试剂盒的显色试剂来测量脂质过氧化物的方法。原则上，该测定利用脂质过氧化物的氧化能力将碘化物转化为碘，碘可在365nm处进行光度测量。使用过氧化氢、叔丁基过氧化氢和异丙苯过氧化氢等过氧化物获得校准曲线。在所测定的有机过氧化物量与产生的碘浓度之间观察到化学计量关系。脂质过氧化物的浓度低至1nmol/ml也能够被测量。证明了能够对分离的低密度脂蛋白的脂质过氧化物进行估计。
