Weber Wilfried, Luzi Stefan, Karlsson Maria, Sanchez-Bustamante Carlota Diaz, Frey Urs, Hierlemann Andreas, Fussenegger Martin
ETH Zurich, Department of Biosystems Science and Engineering, Mattenstrasse 26, CH-4058 Basel, Switzerland.
Nucleic Acids Res. 2009 Mar;37(4):e33. doi: 10.1093/nar/gkp014. Epub 2009 Feb 3.
Electric signal processing has evolved to manage rapid information transfer in neuronal networks and muscular contraction in multicellular organisms and controls the most sophisticated man-built devices. Using a synthetic biology approach to assemble electronic parts with genetic control units engineered into mammalian cells, we designed an electric power-adjustable transcription control circuit able to integrate the intensity of a direct current over time, to translate the amplitude or frequency of an alternating current into an adjustable genetic readout or to modulate the beating frequency of primary heart cells. Successful miniaturization of the electro-genetic devices may pave the way for the design of novel hybrid electro-genetic implants assembled from electronic and genetic parts.
电信号处理已经发展到能够管理神经元网络中的快速信息传递以及多细胞生物中的肌肉收缩，并控制最复杂的人造设备。通过合成生物学方法将电子部件与工程化到哺乳动物细胞中的遗传控制单元组装在一起，我们设计了一种电力可调转录控制电路，它能够整合直流电随时间的强度，将交流电的幅度或频率转化为可调的基因读数，或者调节原代心脏细胞的跳动频率。电-遗传装置的成功小型化可能为设计由电子和遗传部件组装而成的新型混合电-遗传植入物铺平道路。
