Varga A, Schober K E, Walker W L, Lakritz J, Michael Rings D
Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA.
J Vet Intern Med. 2009 Mar-Apr;23(2):359-65. doi: 10.1111/j.1939-1676.2009.0256.x. Epub 2009 Feb 3.
Commercially available cardiac troponin I (cTnI) assays developed for use in humans have not yet been validated for use in cattle.
The ADVIA Centaur TnI-Ultra immunoassay can be used for the detection of bovine cTnI. In healthy cattle, serum cTnI is undetectable or is present only in trace amounts.
Purified bovine cTnI and cTnI-free bovine serum were used for the evaluation of assay performance including intra- and inter-assay precision, sensitivity, interference, linearity, and recovery. Effects of storage at 23, 4, -20, and -80 degrees C for 2 days, and at -20 and -80 degrees C for 7 and 14 days and repeated freeze-thaw cycles on recovery of cTnI were analyzed. Serum cTnI concentrations in 30 healthy dairy cows were determined.
Intra- and inter-assay precisions (mean +/- SD) were 4.48 +/- 2.26 and 13.36 +/- 6.59%, respectively. The assay demonstrated linearity at 0.5, 2, 15, and 30 ng/mL cTnI. Mean recovery was 100.81, 85.26, 87.72, and 114.42%, respectively. Skeletal muscle homogenate added to serum of known cTnI concentration did not alter the concentration of the analyte (P > .05). Concentration of cTnI significantly decreased when samples were stored at 4 and 23 degrees C for 2 days (P < .05). Repeated freeze-thaw cycles and storage at -20 degrees C for 7 days had no significant influence on cTnI concentration (P > .05). Serum cTnI concentration in healthy cattle was <or=0.03 ng/mL.
ADVIA Centaur can be used reliably for the detection of serum cTnI concentration in cattle.
为人类使用而开发的市售心肌肌钙蛋白I（cTnI）检测方法尚未在牛身上得到验证。
ADVIA Centaur TnI-Ultra免疫分析可用于检测牛cTnI。在健康牛中，血清cTnI检测不到或仅以微量存在。
使用纯化的牛cTnI和不含cTnI的牛血清评估检测性能，包括批内和批间精密度、灵敏度、干扰、线性和回收率。分析了在23、4、-20和-80摄氏度下储存2天，以及在-20和-80摄氏度下储存7天和14天及反复冻融循环对cTnI回收率的影响。测定了30头健康奶牛的血清cTnI浓度。
批内和批间精密度（均值±标准差）分别为4.48±2.26%和13.36±6.59%。该检测方法在cTnI浓度为0.5、2、15和30 ng/mL时显示出线性。平均回收率分别为100.81%、85.26%、87.72%和114.42%。向已知cTnI浓度的血清中添加骨骼肌匀浆不会改变分析物的浓度（P>.05）。当样品在4和23摄氏度下储存2天时，cTnI浓度显著降低（P<.05）。反复冻融循环和在-20摄氏度下储存7天对cTnI浓度无显著影响（P>.05）。健康牛的血清cTnI浓度≤0.03 ng/mL。
ADVIA Centaur可可靠地用于检测牛血清中的cTnI浓度。
