Lomas-Francis Christine, Alcantara Denden, Westhoff Connie, Uehlinger Joan, Valvasori Marilia, Castilho Lillian, Reid Marion E
Laboratory of Immunohematology, New York Blood Center, 310 East 67th Street, New York, NY 10065, USA.
Transfusion. 2009 Apr;49(4):719-24. doi: 10.1111/j.1537-2995.2008.02025.x. Epub 2008 Dec 15.
JAL (RH48) is a low-prevalence antigen in the Rh blood group system and anti-JAL has caused hemolytic disease of the newborn. JAL is associated with either a haplotype carrying depressed C and e antigens or one carrying depressed c and e antigens. Blood samples from JAL+ people were tested, published serologic findings were confirmed, serologic studies were extended to include expression of other Rh antigens, and the antibody specificities produced by three sensitized JAL+ probands are reported.
Red blood cell (RBC) samples from 17 (12 probands) JAL+ persons were tested by hemagglutination using standard methods.
RBCs from both the Caucasian JAL+ probands had the (C)(e) haplotype and weakened C, e, hr(B), and hr(S) antigens. JAL+ samples from black persons had the (c)(e) haplotype and expressed weakened c, e, f, V, VS, hr(B), and hr(S) antigens. Plasma from three sensitized c+e+ JAL+ probands contained alloanti-c, alloanti-e, or alloantibody of apparent anti-Rh17 specificity. This study shows that this alloanti-Rh17-like antibody recognizes the high-prevalence antigen antithetical to JAL that has been named CEST.
The presence of the JAL antigen has a quantitative (weakening) effect on the expression of C, e, hr(B), and hr(S) antigens in Caucasian persons and of c, e, f, V, VS, hr(B), and hr(S) antigens in people of black African ancestry. A qualitative effect also was demonstrated by the presence of alloanti-c or alloanti-e in the plasma of two transfused c+e+ patients and by an antibody (anti-CEST) that recognizes the high-prevalence antigen antithetical to JAL.
JAL（RH48）是Rh血型系统中的一种低频率抗原，抗JAL可导致新生儿溶血病。JAL与携带减弱的C和e抗原的单倍型或携带减弱的c和e抗原的单倍型相关。对JAL阳性个体的血样进行了检测，证实了已发表的血清学研究结果，血清学研究扩展至包括其他Rh抗原的表达，并报告了三名致敏JAL阳性先证者产生的抗体特异性。
采用标准方法通过血凝试验检测了17名（12名先证者）JAL阳性个体的红细胞（RBC）样本。
白种人JAL阳性先证者的红细胞均具有（C）（e）单倍型，且C、e、hr（B）和hr（S）抗原减弱。黑人的JAL阳性样本具有（c）（e）单倍型，且c、e、f、V、VS、hr（B）和hr（S）抗原表达减弱。三名致敏的c+e+JAL阳性先证者的血浆中含有同种抗-c、同种抗-e或具有明显抗Rh17特异性的同种抗体。本研究表明，这种类抗Rh17抗体识别与JAL相对的高频率抗原，该抗原已被命名为CEST。
JAL抗原的存在对白种人中C、e、hr（B）和hr（S）抗原以及非洲黑人后裔中c、e、f、V、VS、hr（B）和hr（S）抗原的表达具有定量（减弱）作用。两名接受输血的c+e+患者血浆中存在同种抗-c或同种抗-e以及识别与JAL相对的高频率抗原的抗体（抗CEST）也证明了其定性作用。
