Kallimanis A, Kavakiotis K, Perisynakis A, Spröer C, Pukall R, Drainas C, Koukkou A I
Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece.
Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):275-9. doi: 10.1099/ijs.0.000984-0.
A novel phenanthrene-degrading bacterium, designated strain Sphe3(T), was isolated from a creosote-contaminated soil in Greece. Cells were non-motile, Gram-positive, aerobic, and rod- to coccus-shaped. The strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. Optimal growth occurred at 30 degrees C. The G+C content of the DNA was 65.7 mol%. The polar lipid pattern of strain Sphe3(T) consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The dominant fatty acids were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0), C(16 : 0) and anteiso-C(17 : 0), representing >86 % of the total fatty acids. The predominant isoprenoid quinone of strain Sphe3(T) was menaquinone-8 (MK-8). Based on 16S rRNA gene sequence analysis, strain Sphe3(T) showed 99 and 98.9 % similarity to the type strains of Arthrobacter oxydans and Arthrobacter polychromogenes, respectively. Strain Sphe3(T) showed 91 % similarity to homologues of A. oxydans and A. polychromogenes based on recA gene sequence analysis. Based on 16S rRNA and recA gene sequence analysis and DNA-DNA hybridization analysis, as well as physiological and chemotaxonomic characteristics, it is concluded that strain Sphe3(T) represents a novel species of the genus Arthrobacter, for which the name Arthrobacter phenanthrenivorans sp. nov. is proposed. The type strain is Sphe3(T) (=DSM 18606(T) =LMG 23796(T)).
从希腊受杂酚油污染的土壤中分离出一株新型菲降解细菌，命名为菌株Sphe3(T)。细胞无运动性，革兰氏阳性，好氧，呈杆状至球状。该菌株是根据在喷洒菲的琼脂平板上形成透明圈而分离得到的。最适生长温度为30℃。DNA的G+C含量为65.7 mol%。菌株Sphe3(T)的极性脂质模式由磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油组成。主要脂肪酸为异-C(15 : 0)、anteiso-C(15 : 0)、异-C(16 : 0)、C(16 : 0)和anteiso-C(17 : 0)，占总脂肪酸的>86%。菌株Sphe3(T)的主要类异戊二烯醌为甲基萘醌-8（MK-8）。基于16S rRNA基因序列分析，菌株Sphe3(T)与氧化节杆菌和多色节杆菌的模式菌株分别具有99%和98.9%的相似性。基于recA基因序列分析，菌株Sphe3(T)与氧化节杆菌和多色节杆菌的同源物具有91%的相似性。基于16S rRNA和recA基因序列分析以及DNA-DNA杂交分析，以及生理和化学分类特征，得出结论：菌株Sphe3(T)代表节杆菌属的一个新物种，为此提出新名称菲降解节杆菌（Arthrobacter phenanthrenivorans sp. nov.）。模式菌株为Sphe3(T)（=DSM 18606(T)=LMG 23796(T)）。
