Zhou Yu, Wei Wei, Wang Xu, Lai Ren
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):259-63. doi: 10.1099/ijs.0.000695-0.
A novel actinomycete strain, designated CW 108(T), was isolated from a forest soil in Anhui Province, China. The cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30-37 degrees C and pH 6.0-8.0. Chemotaxonomically, the peptidoglycan type was A3alpha, the cell-wall sugars contained galactose, mannose and ribose, the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylmonomethylethanolamine, the major fatty acids were ai-C(15 : 0), i-C(15 : 0) and ai-C(17 : 0) and the predominant respiratory quinone was MK-9(H(2)), with a moderate amount of MK-8(H(2)) and a minor amount of MK-6(H(2)). The G+C content of the genomic DNA was 71.1 mol%. Phylogenetic analysis showed that strain CW 108(T) formed a deeply separated lineage in the family Micrococcaceae with Arthrobacter atrocyaneus DSM 20127(T). The 16S rRNA gene sequence similarity between strain CW 108(T) and Arthrobacter atrocyaneus DSM 20127(T) was 99.4 %; sequence similarities between these strains and representatives of other species in the family Micrococcaceae with validly published names were lower than 95.2 %. DNA-DNA hybridization showed a level of relatedness of 52.2 % between the two neighbours. Based on the results of our polyphasic taxonomic study, strain CW 108(T) and Arthrobacter atrocyaneus DSM 20127(T) should be assigned to two different species of a novel genus within the family Micrococcaceae, for which the names Sinomonas flava gen. nov., sp. nov. and Sinomonas atrocyanea comb. nov. are proposed. The type strain of Sinomonas flava is CW 108(T) (=LMG 24447(T) =KCTC 19388(T) =CCTCC AB 207194(T)) and the type strain of Sinomonas atrocyanea is DSM 20127(T) =JCM 1329(T) =CGMCC 1.1891(T) =ATCC 13752(T).
从中国安徽省的森林土壤中分离出一株新型放线菌菌株，命名为CW 108(T)。该菌株细胞严格需氧，不运动，呈弯曲杆状。该菌株在30 - 37℃和pH 6.0 - 8.0条件下生长最佳。化学分类学研究表明，其肽聚糖类型为A3α，细胞壁糖包含半乳糖、甘露糖和核糖，极性脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和磷脂酰单甲基乙醇胺，主要脂肪酸为anteiso-C(15 : 0)、iso-C(15 : 0)和anteiso-C(17 : 0)，主要呼吸醌为MK-9(H₂)，同时含有适量的MK-8(H₂)和少量的MK-6(H₂)。基因组DNA的G + C含量为71.1 mol%。系统发育分析表明，菌株CW 108(T)在微球菌科中与蓝黑节杆菌DSM 20127(T)形成一个深度分离的分支。菌株CW 108(T)与蓝黑节杆菌DSM 20127(T)的16S rRNA基因序列相似性为99.4%；这些菌株与微球菌科中其他有效发表种的代表菌株之间的序列相似性低于95.2%。DNA - DNA杂交显示这两个近缘菌株之间的相关性水平为52.2%。基于我们多相分类研究的结果，菌株CW 108(T)和蓝黑节杆菌DSM 20127(T)应被归入微球菌科内一个新属的两个不同种，为此提出新属名中华单胞菌属（Sinomonas）、新种名黄色中华单胞菌（Sinomonas flava）及新组合名蓝黑中华单胞菌（Sinomonas atrocyanea）。黄色中华单胞菌的模式菌株为CW 108(T)（=LMG 24447(T)=KCTC 19388(T)=CCTCC AB 207194(T)），蓝黑中华单胞菌的模式菌株为DSM 20127(T)=JCM 1329(T)=CGMCC 1.1891(T)=ATCC 13752(T)。
