Silva C G, Raulino R J, Cerqueira D M, Mannarino S C, Pereira M D, Panek A D, Silva J F M, Menezes F S, Eleutherio E C A
Departamento de Bioquímica, Instituto de Química, UFRJ, Rio de Janeiro, Brazil.
Phytomedicine. 2009 Aug;16(8):761-7. doi: 10.1016/j.phymed.2008.12.019. Epub 2009 Feb 5.
Reactive oxygen species (ROS) are thought to underline the process of ageing and the pathogenicity of various diseases, such as neurodegenerative disorders and cancer. The use of traditional medicine is widespread and plants still present a large source of natural antioxidants that might serve as leads for the development of novel drugs. In this paper, the alcoholic extract from leaves of Hyptis fasciculata, a Brazilian medicinal plant, and isoquercitrin, a flavonoid identified in this species, showed to be active as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavengers. The extract of Hyptis fasciculata and isoquercitrin were also able to increase tolerance of the eukaryotic microorganism Saccharomyces cerevisiae to both hydrogen peroxide and menadione, a source of superoxide. Cellular protection was correlated with a decrease in oxidative stress markers, such as levels of ROS, protein carbonylation and lipid peroxidation, confirming the antioxidant potential of Hyptis fasciculata and isoquercitrin.
活性氧（ROS）被认为是衰老过程以及各种疾病（如神经退行性疾病和癌症）致病性的潜在因素。传统医学的应用广泛，植物仍然是天然抗氧化剂的重要来源，这些抗氧化剂可能成为开发新药的先导。在本文中，巴西药用植物束花山香茶（Hyptis fasciculata）叶片的乙醇提取物以及该物种中鉴定出的类黄酮异槲皮苷，显示出作为1,1-二苯基-2-苦基肼（DPPH）自由基清除剂具有活性。束花山香茶提取物和异槲皮苷还能够提高真核微生物酿酒酵母对过氧化氢和超氧化物来源甲萘醌的耐受性。细胞保护作用与氧化应激标志物（如ROS水平、蛋白质羰基化和脂质过氧化）的降低相关，证实了束花山香茶和异槲皮苷的抗氧化潜力。
