Centro Trasfusionale A-ULS 14.
Blood Transfus. 2007 Jul;5(3):130-3. doi: 10.2450/2007.0003-07.
Transfusion-associated bacterial infections are a quite frequent collateral effect of administration of platelet concentrates (PC). We carried out a microbiological surveillance of bacterial contamination of apheresis platelet concentrates by studying microbial flora on donors' arms before and after skin disinfection, through blood cultures with the diversion volume and with the PC.
Platelet aphereses were carried out using two Haemonetics MCS+ instruments. Cutaneous swabs were examined by the direct plate technique and blood cultures were performed using Bact/ALERT aerobic bottles. In the 5 years from January 2001 to December 2005 we tested 481 PC.
Cutaneous swabs showed significant bacterial growth in 89% of cases before skin disinfection and in 44% after. None of the blood cultures performed on diversion blood was positive, one (0.2%) PC was positive on the fifth day after collection and the presence of a Staphylococcus epidermidis strain was demonstrated.
Our results suggest that the skin disinfection protocol adopted in our structure is not fully satisfactory. The cultures performed on the PC showed a low prevalence of contamination, and the only positive sample was contaminated by a common skin contaminant (S. epidermidis). The culture became positive on the fifth day after collection, but on the second day the PC had been transfused to a patient, without any adverse reaction. In our experience a culture method using Bact/ALERT aerobic bottles was not able to prevent transfusion of the only contaminated PC identified in this study.
血小板浓缩物（PC）输注相关的细菌感染是其常见的副作用。我们通过对供者手臂在皮肤消毒前后的血液培养（包括分流体积和 PC），研究微生物菌群，对血小板单采术的细菌污染进行了微生物监测。
使用两台 Haemonetics MCS+仪器进行血小板单采术。通过直接平板技术检查皮肤拭子，使用 Bact/ALERT 需氧瓶进行血培养。在 2001 年 1 月至 2005 年 12 月的 5 年内，我们共检测了 481 份 PC。
皮肤消毒前 89%的情况下皮肤拭子有明显细菌生长，消毒后 44%的情况下有细菌生长。在分流血上进行的所有血培养均为阳性，在采集后第 5 天有 1 份（0.2%）PC 阳性，证明存在表皮葡萄球菌。
我们的结果表明，我们机构采用的皮肤消毒方案并不完全令人满意。对 PC 进行的培养显示污染率较低，唯一的阳性样本被常见的皮肤污染物（表皮葡萄球菌）污染。该培养物在采集后第 5 天呈阳性，但在第 2 天就已经输给了一名患者，没有任何不良反应。根据我们的经验，使用 Bact/ALERT 需氧瓶的培养方法未能阻止本研究中唯一被污染的 PC 的输血。
