Kawabata M, Kubo N, Arashima Y, Yoshida M, Kawano K
Department of Clinical Pathology, Nihon University School of Medicine, Tokyo.
Rinsho Byori. 1991 Aug;39(8):891-4.
Antibodies to a 41,000 (41 kD) polypeptide in flagella of Borrelia burgdorferi were measured in patients with Lyme disease in Japan by flagellum ELISA. The IgG and IgM Classes of antibodies to a flagellum antigens were detected in the sera as early as 0.5 months after infection. The IgG antibodies continued to exist in their sera for more than one year, while the IgM antibodies quickly faded out from their sera. With respect to a diagnostic specificity of the flagellum ELISA, false positive reactions showing more than 10% were observed in sera with high levels of IgG or IgM, and with anti-syphilis antibody. This method, however, was unaffected by sera with high levels of IgA, rheumatoid factor or anti-nuclear antibody. In three cases of patients with erythema migrans preceded by tick-bite, and treated with antibiotics, seronegative results were observed by a immunoperoxidase (IP) test. Since two of them showed the positive level of IgM antibody by the flagellum ELISA, this method seems to be more sensitive and useful than the IP test for serodiagnosis of the Lyme disease.
通过鞭毛酶联免疫吸附测定法（ELISA）检测了日本莱姆病患者血清中针对伯氏疏螺旋体鞭毛中一种41,000（41kD）多肽的抗体。早在感染后0.5个月就在血清中检测到了针对鞭毛抗原的IgG和IgM类抗体。IgG抗体在患者血清中持续存在一年以上，而IgM抗体则很快从血清中消失。关于鞭毛ELISA的诊断特异性，在IgG或IgM水平较高以及含有抗梅毒抗体的血清中观察到超过10%的假阳性反应。然而，该方法不受高水平IgA、类风湿因子或抗核抗体血清的影响。在3例有蜱叮咬史且出现游走性红斑并接受抗生素治疗的患者中，免疫过氧化物酶（IP）试验结果为血清阴性。由于其中2例通过鞭毛ELISA显示IgM抗体呈阳性水平，因此该方法在莱姆病血清诊断中似乎比IP试验更敏感、更有用。
