非梗阻性无精子症患者中微小RNA表达的改变。
Altered microRNA expression in patients with non-obstructive azoospermia.
作者信息
Lian Jie, Zhang Xiansheng, Tian Hui, Liang Ning, Wang Yong, Liang Chaozhao, Li Xin, Sun Fei
机构信息
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, PR China.
出版信息
Reprod Biol Endocrinol. 2009 Feb 11;7:13. doi: 10.1186/1477-7827-7-13.
BACKGROUND
MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility.
METHODS
In this study, the miRNA expression profiles of testes of patients with non-obstructive azoospermia (NOA) and normal controls were performed by using microarray technologies.
RESULTS
Altered microRNA expression in NOA patients was found, with 154 differentially down-regulated and 19 up-regulated miRNAs. These findings have been confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) assays on select miRNAs, including miR-302a, miR-491-3p, miR-520d-3p and miR-383. Several down-regulated miRNA clusters in patients with NOA were identified, such as the oncogenic potential of the mir-17-92 cluster and mir-371,2,3 cluster.
CONCLUSION
This is the first report that the expression of miRNAs is altered in testicular tissues of patients with NOA, suggesting a role of miRNAs in regulating spermatogenesis in human males.
背景
微小RNA（miRNA）是一类小的非编码RNA分子，被认为在精子发生过程中发挥重要作用。然而，关于参与不育症的人类睾丸中miRNA的表达模式或功能知之甚少。
方法
在本研究中，利用微阵列技术对非梗阻性无精子症（NOA）患者和正常对照者的睾丸进行miRNA表达谱分析。
结果
发现NOA患者中miRNA表达发生改变，有154个miRNA差异下调，19个miRNA上调。这些发现已通过对包括miR-302a、miR-491-3p、miR-520d-3p和miR-383在内的选定miRNA进行实时逆转录-聚合酶链反应（RT-PCR）分析得到证实。在NOA患者中鉴定出几个下调的miRNA簇，如mir-17-92簇和mir-371,2,3簇的致癌潜力。
结论
这是首次报道NOA患者睾丸组织中miRNA表达发生改变，提示miRNA在调节人类男性精子发生中起作用。
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