Jones Sara E, Versalovic James
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
BMC Microbiol. 2009 Feb 11;9:35. doi: 10.1186/1471-2180-9-35.
Commensal-derived probiotic bacteria inhibit enteric pathogens and regulate host immune responses in the gastrointestinal tract, but studies examining specific functions of beneficial microbes in the context of biofilms have been limited in scope.
Lactobacillus reuteri formed biofilms that retained functions potentially advantageous to the host including modulation of cytokine output and the production of the antimicrobial agent, reuterin. Immunomodulatory activities of biofilms were demonstrated by the abilities of specific L. reuteri strains to suppress human TNF production by LPS-activated monocytoid cells. Quantification of the antimicrobial glycerol derivative, reuterin, was assessed in order to document the antipathogenic potential of probiotic biofilms. L. reuteri biofilms differed in the quantities of reuterin secreted in this physiological state.
L. reuteri biofilms secreted factors that confer specific health benefits such as immunomodulation and pathogen inhibition. Future probiotic selection strategies should consider a strain's ability to perform beneficial functions as a biofilm.
共生来源的益生菌可抑制肠道病原体并调节胃肠道中的宿主免疫反应，但在生物膜背景下研究有益微生物特定功能的研究范围有限。
罗伊氏乳杆菌形成的生物膜保留了对宿主可能有利的功能，包括调节细胞因子输出和产生抗菌剂罗伊氏菌素。特定罗伊氏乳杆菌菌株抑制脂多糖激活的单核细胞样细胞产生人肿瘤坏死因子的能力证明了生物膜的免疫调节活性。为了记录益生菌生物膜的抗病原体潜力，对抗菌甘油衍生物罗伊氏菌素进行了定量分析。罗伊氏乳杆菌生物膜在这种生理状态下分泌的罗伊氏菌素数量有所不同。
罗伊氏乳杆菌生物膜分泌的因子具有免疫调节和病原体抑制等特定的健康益处。未来的益生菌选择策略应考虑菌株作为生物膜发挥有益功能的能力。
