非晶态硫化亚铁作为厌氧菌培养的还原剂。
Amorphous ferrous sulfide as a reducing agent for culture of anaerobes.
作者信息
Brock T D, Od'ea K
出版信息
Appl Environ Microbiol. 1977 Feb;33(2):254-6. doi: 10.1128/aem.33.2.254-256.1977.
Abstract
Amorphous ferrous sulfide, prepared by reacting ferrous ammonium sulfate and sodium sulfide, is an excellent reducing agent for the culture of anaerobes. It reduces resazurin and reacts much more rapidly with O2 than does either soluble sulfide (HS)- or cysteine. One of the end products of the oxidation of ferrous sulfide with O2 is red and serves as an indicator for the oxygen contamination of a culture medium. Amorphous ferrous sulfide served as a suitable reducing agent for the growth of species of Methanobacterium or Clostridium. Its use is recommended for enrichment or culture of anaerobes (e.g. autotrophs, fermentative organisms) from sediments and other habitats were organic reducing agents are undesirable and where soluble sulfide might be toxic.
摘要
通过硫酸亚铁铵与硫化钠反应制备的无定形硫化亚铁，是用于厌氧菌培养的优良还原剂。它能使刃天青还原，并且与氧气的反应比可溶性硫化物（HS）-或半胱氨酸快得多。硫化亚铁与氧气氧化的最终产物之一是红色的，可作为培养基被氧气污染的指示剂。无定形硫化亚铁是甲烷杆菌属或梭菌属物种生长的合适还原剂。推荐将其用于从沉积物和其他生境中富集或培养厌氧菌（如自养菌、发酵型生物），在这些生境中有机还原剂不合适且可溶性硫化物可能有毒。
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本文引用的文献
1
Acidic mine drainage: the rate-determining step.酸性矿山排水：速率控制步骤。
Science. 1970 Feb 20;167(3921):1121-3. doi: 10.1126/science.167.3921.1121.
2
A selective enrichment method for Gallionella ferruginea.一种针对铁锈盖菌的选择性富集方法。
J Bacteriol. 1957 Sep;74(3):344-9. doi: 10.1128/jb.74.3.344-349.1957.
3
Microbial life at 90 C: the sulfur bacteria of Boulder Spring.90摄氏度下的微生物生命：博尔德温泉的硫细菌。
J Bacteriol. 1971 Jul;107(1):303-14. doi: 10.1128/jb.107.1.303-314.1971.
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