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使用毛细管电泳和微流控设备进行单细胞分析的最新进展。

Recent advances in single-cell analysis using capillary electrophoresis and microfluidic devices.

作者信息

Huang Wei-Hua, Ai Feng, Wang Zong-Li, Cheng Jie-Ke

机构信息

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 15;866(1-2):104-22. doi: 10.1016/j.jchromb.2008.01.030. Epub 2008 Feb 2.

Abstract

Cells are the fundamental unit of life, and studies on cell contribute to reveal the mystery of life. However, since variability exists between individual cells even in the same kind of cells, increased emphasis has been put on the analysis of individual cells for getting better understanding on the organism functions. During the past two decades, various techniques have been developed for single-cell analysis. Capillary electrophoresis is an excellent technique for identifying and quantifying the contents of single cells. The microfluidic devices afford a versatile platform for single-cell analysis owing to their unique characteristics. This article provides a review on recent advances in single-cell analysis using capillary electrophoresis and microfluidic devices; focus areas to be covered include sampling techniques, detection methods and main applications in capillary electrophoresis, and cell culture, cell manipulation, chemical cytometry and cellular physiology on microfluidic devices.

摘要

细胞是生命的基本单位,对细胞的研究有助于揭示生命的奥秘。然而,即使是同一种细胞,个体细胞之间也存在差异,因此为了更好地理解生物体功能,人们越来越重视对单个细胞的分析。在过去的二十年里,已经开发出了各种用于单细胞分析的技术。毛细管电泳是一种用于识别和定量单个细胞内容物的优秀技术。微流控装置因其独特的特性,为单细胞分析提供了一个多功能平台。本文综述了利用毛细管电泳和微流控装置进行单细胞分析的最新进展;涵盖的重点领域包括毛细管电泳中的采样技术、检测方法和主要应用,以及微流控装置上的细胞培养、细胞操作、化学细胞术和细胞生理学。

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