[红豆杉中双黄酮的结构鉴定及紫杉醇的测定]
[Structrue identification of biflavones and determination of Taxol from Taxus Madia].
作者信息
Liu Xiang-Qian, Zhang Xiao-Dan, Zhu Yu-Lin, Shin Bum-Young, Wu Shi-Xu
机构信息
Department of Pharmaceutical Engineering, Central South University, Changsha 410083, China.
出版信息
Zhong Yao Cai. 2008 Oct;31(10):1498-501.
OBJECTIVE
To study the flavonoids from Taxus Madia and determine the content of Taxol from different parts of Taxus Madia.
METHODS
Various column chromatographic techniques were applied for isolation and purification. The structrues were elucidated by physico-chemical property and spectral evidence. The analysis of Taxol was carried out on C18 (150 mm x 4.6 mm i. d. 5 microm). The mobile phase was MeOH-H2O (3:1). The flow-rate was 0.8 ml/min. The detection wave-length was 228 nm.
RESULTS
Three bifiavonyl compounds were isolated and identified as Sciadopitysin (Compound I), Ginkgetin (Compound II), 7,7",4'-Tri-O-Methylamentoflavone (Compound III). The contents of Taxol from branches and leaves, main root, fibrous root was 0.0308 mg/g, 0.02191 mg/g, 0.01983 mg/g respectively.
CONCLUSION
Compound I-III are obtained from Taxus Madia for the first time. The contents of Taxol is the highest in the part of branches and leaves, and then is the main root, the lowest is the fibrous root.
目的
研究马迪亚红豆杉中的黄酮类化合物，并测定马迪亚红豆杉不同部位的紫杉醇含量。
方法
采用多种柱色谱技术进行分离纯化。通过理化性质和光谱证据阐明结构。紫杉醇的分析在C18柱（150 mm×4.6 mm内径，5μm）上进行。流动相为甲醇 - 水（3:1）。流速为0.8 ml/min。检测波长为228 nm。
结果
分离并鉴定出三种双黄酮化合物，分别为穗花杉双黄酮（化合物I）、银杏双黄酮（化合物II）、7,7",4'-三 - O - 甲基穗花杉双黄酮（化合物III）。枝叶、主根、须根中紫杉醇的含量分别为0.0308 mg/g、0.02191 mg/g、0.01983 mg/g。
结论
化合物I - III首次从马迪亚红豆杉中获得。紫杉醇含量在枝叶部分最高，其次是主根，须根最低。
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