对氧磷酶1（PON1）5'-调控区多态性、PON1活性与缺血性中风之间的关联
Association between PON1 5'-regulatory region polymorphisms, PON1 activity and ischemic stroke.
作者信息
Kim No Soo, Kang Byoung Kab, Cha Min Ho, Oh Se-Mi, Ko Mi Mi, Bang Ok-Sun
机构信息
Department of Medical Research, Korea Institute of Oriental Medicine, 461-24 Jeonmin-dong, Yuseong-gu, Daejeon, 305-811, Republic of Korea.
出版信息
Clin Biochem. 2009 Jun;42(9):857-63. doi: 10.1016/j.clinbiochem.2009.02.008. Epub 2009 Feb 20.
OBJECTIVES
Paraoxonase I (PON1) was known as a risk factor for cerebrovascular diseases. This study assessed the association of single nucleotide polymorphisms (SNPs) in the PON1 5'-regulatory region with ischemic stroke and serum PON1 activity.
DESIGN AND METHODS
Study subjects consisted of 418 healthy controls and 86 ischemic stroke patients with small vessel occlusion. SNPs were identified by DNA sequencing and a primer extension-based method.
RESULTS
Among 10 identified SNPs, only -1434GG genotype was observed with a lower frequency in patients on borderline statistical significance (OR(95% CI), 0.297(0.083-1.060), p=0.0615). However, haplotype analysis in a dominant model revealed that ht2 was observed with a significantly lower frequency in patients (OR(95% CI), 0.390(0.153-0.991), p=0.0477). Both C(-1434)G mutation and ht2 distribution were associated with serum PON1 activity.
CONCLUSION
Our results suggest that haplotypes observed in the PON1 5'-regulatory region should be considered as risk factors for ischemic stroke.
目的
对氧磷酶I（PON1）被认为是脑血管疾病的一个风险因素。本研究评估了PON1 5'-调控区单核苷酸多态性（SNP）与缺血性中风及血清PON1活性之间的关联。
设计与方法
研究对象包括418名健康对照者和86名小血管闭塞性缺血性中风患者。通过DNA测序和基于引物延伸的方法鉴定SNP。
结果
在鉴定出的10个SNP中，仅-1434GG基因型在患者中的观察频率较低，具有临界统计学意义（比值比[95%可信区间]，0.297[0.083 - 1.060]，p = 0.0615）。然而，显性模型的单倍型分析显示，单倍型ht2在患者中的观察频率显著较低（比值比[95%可信区间]，0.390[0.153 - 0.991]，p = 0.0477）。C（-1434）G突变和ht2分布均与血清PON1活性相关。
结论
我们的结果表明，PON1 5'-调控区观察到的单倍型应被视为缺血性中风的风险因素。
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