Bray M, Lai C J
Molecular Viral Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Virology. 1991 Nov;185(1):505-8. doi: 10.1016/0042-6822(91)90809-p.
We have constructed recombinant vaccinia viruses that express the premembrane (pre-M), membrane (M), or the cleaved, residual portion of pre-M (non-M) proteins of dengue 4 virus, or the pre-M, non-M, or envelope (E) proteins of dengue 2 virus, to evaluate their ability to induce protective immunity in mice. Cells infected with these recombinants make proteins of expected size. Mice immunized with recombinants expressing dengue 4 pre-M or M were protected against subsequent dengue 4 encephalitis challenge, but non-M was not protective. However, a recombinant that expressed both pre-M and E as a polyprotein gave solid protection, while the simultaneous administration of the two recombinants expressing pre-M and E gave a significant level of protection. Pre-M and M function as antigens eliciting a protective immune response, and the combination of pre-M plus E is more protective than E alone.
我们构建了重组痘苗病毒，这些病毒表达登革4型病毒的前膜（pre-M）、膜（M）蛋白，或pre-M的裂解后残余部分（非M）蛋白，以及登革2型病毒的pre-M、非M或包膜（E）蛋白，以评估它们在小鼠中诱导保护性免疫的能力。感染这些重组体的细胞能产生预期大小的蛋白质。用表达登革4型pre-M或M的重组体免疫的小鼠对随后的登革4型脑炎攻击具有抵抗力，但非M则无保护作用。然而，一个表达pre-M和E作为多聚蛋白的重组体提供了可靠的保护，而同时给予表达pre-M和E的两种重组体也提供了显著水平的保护。pre-M和M作为引发保护性免疫反应的抗原发挥作用，并pre-M加E的组合比单独的E更具保护作用。
