托马霉素（SJG-136单体类似物）生物合成基因簇的克隆与表征
Cloning and characterization of the biosynthetic gene cluster for tomaymycin, an SJG-136 monomeric analog.
作者信息
Li Wei, Chou ShenChieh, Khullar Ankush, Gerratana Barbara
机构信息
Department of Chemistry and Biochemistry, Bldg. 091, University of Maryland, College Park, MD 20742, USA.
出版信息
Appl Environ Microbiol. 2009 May;75(9):2958-63. doi: 10.1128/AEM.02325-08. Epub 2009 Mar 6.
Abstract
Tomaymycin produced by Streptomyces achromogenes is a naturally produced pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. The gene cluster analysis reveals a novel biosynthetic pathway for the anthranilate moiety of PBDs. Gene replacement and chemical complementation studies were used to confirm the proposed biosynthetic pathway.
摘要
由产色链霉菌产生的托马霉素是一种天然产生的吡咯并苯二氮䓬（PBD）。已鉴定并测序了托马霉素的生物合成基因簇。基因簇分析揭示了PBDs邻氨基苯甲酸部分的一条新的生物合成途径。采用基因置换和化学互补研究来证实所提出的生物合成途径。
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