新型回归热螺旋体在蝙蝠 tick Carios kelleyi 的中肠、coxal 液和唾液腺中的特征。
Characterization of a novel relapsing fever spirochete in the midgut, coxal fluid, and salivary glands of the bat tick Carios kelleyi.
机构信息
Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
出版信息
Vector Borne Zoonotic Dis. 2009 Dec;9(6):643-7. doi: 10.1089/vbz.2008.0177.
Bat ticks, Carios kelleyi, from Iowa were examined for the presence of relapsing fever group borreliae. A novel spirochete was characterized by DNA sequence analysis of polymerase chain reaction amplicons for the 16S rRNA, flaB, and glpQ genes in either triturated tick pools or single ticks. All loci and the concatenated DNA sequence of 3,289 bases identified the Carios bacterium as a relapsing fever spirochete most closely related to, but distinct from, Borrelia turicatae. Spirochetes reactive with a Borrelia-specific monoclonal antibody were observed microscopically in the coxal fluid and salivary glands from one tick. These data confirm the presence of a novel species of relapsing fever spirochete in bat ticks and the potential for new enzootic foci for endemic relapsing fever that warrants further investigation. The name Borrelia johnsonii is proposed for this novel spirochete in honor of Dr. Russell C. Johnson.
从爱荷华州采集的 Carios kelleyi 蝙蝠蜱进行了检测，以确定是否存在回归热群螺旋体。通过聚合酶链反应扩增 16S rRNA、flaB 和 glpQ 基因的 DNA 序列分析，对在研磨蜱池或单个蜱中鉴定出的新型螺旋体进行了特征描述。所有基因座和 3289 个碱基的串联 DNA 序列将 Carios 细菌鉴定为回归热螺旋体，与 Borrelia turicatae 密切相关，但又有所不同。从一只蜱的 Coxal 液和唾液腺中观察到与 Borrelia 特异性单克隆抗体反应的螺旋体。这些数据证实了蝙蝠蜱中存在一种新型回归热螺旋体，存在地方性回归热新的地方性流行焦点，值得进一步研究。为了纪念 Russell C. Johnson 博士，提议将这种新型螺旋体命名为 Borrelia johnsonii。
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