Johanson G, Johnsson S
Division of Work and Environmental Physiology, National Institute of Occupational Health, Solna, Sweden.
Arch Toxicol. 1991;65(5):433-5. doi: 10.1007/BF02284269.
Venous blood samples from five male volunteers exposed to 20 ppm 2-butoxyethanol (BE) for 2 h were collected at 0, 2, 4, and 6 h from the start of exposure and analyzed by gas chromatography after simultaneous ion-pair extraction and derivatization with pentafluorobenzyl bromide. Butoxyacetic acid (BAA), a major metabolite of BE, was found in all samples except those collected prior to exposure. This is the first time to our knowledge that the analysis of BAA in human blood has been reported. Concentrations of BAA in blood ranged from 22 to 60 microM. These concentrations were about two orders of magnitude lower than those causing swelling and hemolysis of human erythrocytes in vitro. The BAA blood level peaked after 2-4 h. The decrease between 4 and 6 h indicates an average half-time of BAA in blood of about 4 h, which is in accordance with previously observed half-times in urine. The low renal clearance of BAA (22-39 ml/min) indicates extensive binding to blood proteins and poor tubular secretion of the substance. Binding of BAA to blood components is also indicated by the low apparent volume of distribution of approximately 15l.
从五名男性志愿者身上采集静脉血样本，他们暴露于20 ppm的2-丁氧基乙醇（BE）中2小时，在暴露开始后的0、2、4和6小时采集样本，并在同时进行离子对萃取并用五氟苄基溴衍生化后通过气相色谱法进行分析。除暴露前采集的样本外，在所有样本中均发现了BE的主要代谢产物丁氧基乙酸（BAA）。据我们所知，这是首次报道对人血中BAA的分析。血液中BAA的浓度范围为22至60微摩尔。这些浓度比在体外引起人红细胞肿胀和溶血的浓度低约两个数量级。BAA血药浓度在2至4小时后达到峰值。4至6小时之间的下降表明BAA在血液中的平均半衰期约为4小时，这与先前在尿液中观察到的半衰期一致。BAA的低肾清除率（22 - 39毫升/分钟）表明其与血液蛋白广泛结合且该物质的肾小管分泌较差。约15升的低表观分布容积也表明BAA与血液成分结合。
