Lahmar Ibtissem, Abou-Bacar Ahmed, Abdelrahman Tamer, Guinard Marie, Babba Hamouda, Ben Yahia Salim, Kairallah Moncef, Speeg-Schatz Claude, Bourcier Tristan, Sauer Arnaud, Villard Odile, Pfaff Alexander W, Mousli Marc, Garweg Justus G, Candolfi Ermanno
Institut de Parasitologie et de Pathologie Tropicale, Université de Strasbourg, Strasbourg, France.
J Infect Dis. 2009 Apr 15;199(8):1239-49. doi: 10.1086/597478.
Uveitis is a major cause of visual impairment throughout the world. Analysis of cytokine profiles in aqueous humor specimens may provide insight into the physiopathological processes that underly retinal damage in this context.
Using a multiplex assay, we determined the concentrations of 17 cytokines and chemokines in aqueous humor specimens obtained from patients with ocular toxoplasmosis or viral uveitis and compared these concentrations with those in specimens obtained from patients with noninfectious intermediate uveitis or cataract.
Five mediators (interleukin [IL]-8, monocyte chemoattractant protein-1, tumor necrosis factor-alpha, IL-4, and IL-10) were detected in >50% of patients in all groups. In contrast, IL-5 and IL-12 were specific for ocular toxoplasmosis, and granulocyte monocyte colony-stimulating factor and IL-1 were specific for viral uveitis; these mediators could present specific markers for diagnostic purposes. Interferon-gamma, IL-6, and macrophage inflammatory protein-1beta were common markers of ocular toxoplasmosis and viral uveitis. IL-17 was a common marker of ocular toxoplasmosis and intermediate uveitis.
We found specific cytokine profiles for each type of uveitis, with large interindividual variations and no etiological or clinical correlations. Ocular cytokine mapping contributes to a better understanding of the physiopathology of specific forms of uveitis and provides guidance for new targeted treatment.
葡萄膜炎是全球视力损害的主要原因。分析房水标本中的细胞因子谱可能有助于深入了解在此情况下视网膜损伤的生理病理过程。
我们采用多重检测法测定了从眼弓形虫病或病毒性葡萄膜炎患者获取的房水标本中17种细胞因子和趋化因子的浓度，并将这些浓度与从非感染性中间葡萄膜炎或白内障患者获取的标本中的浓度进行比较。
在所有组中，超过50%的患者检测到5种介质（白细胞介素[IL]-8、单核细胞趋化蛋白-1、肿瘤坏死因子-α、IL-4和IL-10）。相比之下，IL-5和IL-12是眼弓形虫病特有的，粒细胞单核细胞集落刺激因子和IL-1是病毒性葡萄膜炎特有的；这些介质可作为诊断的特异性标志物。干扰素-γ、IL-6和巨噬细胞炎性蛋白-1β是眼弓形虫病和病毒性葡萄膜炎的常见标志物。IL-17是眼弓形虫病和中间葡萄膜炎的常见标志物。
我们发现每种类型的葡萄膜炎都有特定的细胞因子谱，个体间差异很大，且与病因或临床无相关性。眼部细胞因子图谱有助于更好地理解特定形式葡萄膜炎的生理病理学，并为新的靶向治疗提供指导。
