Hsu M T, Jelinek W R
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1631-4. doi: 10.1073/pnas.74.4.1631.
Single-stranded, linear DNA of simian virus 40 (SV40) created by denaturing the endonuclease EcoRI- or Hpa II-generated, linear, double-stranded products from form I DNA of SV40 was analyzed for regions of inverted repeated sequences by visualization with the electron microscope. Six hairpin loops were found at positions 0.11-0.30 (two loops forming a "rabbit ears" structure), 0.47-0.52, 0.63-0.68, 0.70-0.76, and 0.90-0.96. The nucleotide sequences within all of these inverted repeats may be related since the looped regions can crosshybridize with one another and, thus, the SV40 genome may contain regions of interspersed repeated and unique sequences. The map positions of the 3' and 5' ends of the early and late messenger RNAs, as determined by others, lie within regions of inverted repeated sequences. Previously recorded recombination events that occurred either within the SV40 genome or between SV40 DNA and other genomes have apparently occurred frequently at positions of inverted repeated sequences within the SV40 DNA.
通过用电子显微镜观察，对由猿猴病毒40（SV40）的I型DNA经核酸内切酶EcoRI或Hpa II切割产生的线性双链产物变性后得到的单链线性DNA进行分析，以寻找反向重复序列区域。在0.11 - 0.30位置（两个环形成“兔耳”结构）、0.47 - 0.52、0.63 - 0.68、0.70 - 0.76和0.90 - 0.96处发现了六个发夹环。所有这些反向重复序列中的核苷酸序列可能有关联，因为成环区域可以相互交叉杂交，因此SV40基因组可能包含散布的重复序列和独特序列区域。如其他人所确定的，早期和晚期信使RNA的3'和5'末端的图谱位置位于反向重复序列区域内。先前记录的在SV40基因组内或SV40 DNA与其他基因组之间发生的重组事件显然经常发生在SV40 DNA内的反向重复序列位置。
