Ali Md Sultan, Mudagal M P, Goli D
Department of Pharmacology, Acharya & B. M. Reddy College of Pharmacy, Bangalore, Karnataka, India.
Pharmazie. 2009 Feb;64(2):132-6.
The present study was undertaken to evaluate the cardioprotective potential of tetrahydrocurcumin (THC) and rutin in an in vivo rat ischemia-reperfusion (I/R) model of myocardial infarction (MI). Male wistar rats were divided into six groups receiving saline (control MI/R group), vehicle control MI/R group, THC (5 mg kg(-1) and 10 mg kg(-1)) and rutin (5 mg kg(-1) and 10 mg kg(-1)) i.p. injection respectively. At the day of the experiment, each group was subjected to acute ischemia for 30 min by occlusion of the left anterior descending coronary artery (LAD). Thereafter reperfusion was allowed for 4 h. MI/R resulted in significant cardiac necrosis, elevation in lipid peroxidation, elevation in cardiac marker enzymes AST, ALT and decline in antioxidant status catalase, reduced glutathione in the normal control MI/R group and vehicle control MI/R group. Myocardial infarction produced after MI/R was significantly reduced in tetrahydrocurcumin and rutin of the myocardial antioxidant status, infarct size reduction compared to control and vehicle control MI/R group. Furthermore, MI/R induced lipid peroxidation was significantly reduced by tetrahydrocurcumin and rutin. Cardioprotection in the treatment group was probably a result from suppression of oxidative stress. Histopathological examination further confirmed the protective effect of tetrahydrocurcumin and rutin on the MI/R heart.
本研究旨在评估四氢姜黄素（THC）和芦丁在大鼠心肌梗死（MI）体内缺血再灌注（I/R）模型中的心脏保护潜力。将雄性Wistar大鼠分为六组，分别腹腔注射生理盐水（对照MI/R组）、溶剂对照MI/R组、THC（5 mg kg⁻¹和10 mg kg⁻¹）以及芦丁（5 mg kg⁻¹和10 mg kg⁻¹）。在实验当天，通过结扎左冠状动脉前降支（LAD）使每组大鼠急性缺血30分钟。此后进行4小时的再灌注。MI/R导致正常对照MI/R组和溶剂对照MI/R组出现明显的心肌坏死、脂质过氧化升高、心脏标志物酶AST、ALT升高以及抗氧化状态过氧化氢酶、还原型谷胱甘肽下降。与对照和溶剂对照MI/R组相比，四氢姜黄素和芦丁组MI/R后产生的心肌梗死明显减少，心肌抗氧化状态改善，梗死面积减小。此外，四氢姜黄素和芦丁显著降低了MI/R诱导的脂质过氧化。治疗组的心脏保护作用可能是抑制氧化应激的结果。组织病理学检查进一步证实了四氢姜黄素和芦丁对MI/R心脏的保护作用。
