Prickett Claire D, Lister E, Collins Michelle, Trevithick-Sutton C C, Hirst M, Vinson J A, Noble E, Trevithick J R
Department of Biochemistry and Department of Pharmacology and Toxicology, Faculty of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1.
Nonlinearity Biol Toxicol Med. 2004 Oct;2(4):353-70. doi: 10.1080/15401420490900272.
To correlate the oxidative state of postabsorptive blood plasma after consumption of one or three drinks of different beverages with known J-shaped epidemiological risk curves.
DESIGN, INTERVENTIONS, AND MAIN OUTCOME MEASURES: Red wine, lager beer, stout (alcoholic and alcohol-free), with antioxidant activity, and an aqueous solution of alcohol were compared for the plasma antioxidant or pro-oxidant activity in human volunteers following consumption of one or three typical drinks containing equivalent amounts of alcohol (except for an alcohol-free stout used as a control for stout).
One drink of red wine, lager beer, or stout (5% alcohol v/v, and alcohol-free) significantly increased the average antioxidant activity in plasma samples obtained from volunteers averaged over 240 min. Three drinks of red wine, lager beer, or stout (5% alcohol v/v, and alcohol-free) significantly increased the average pro-oxidant activity in plasma samples obtained from volunteers averaged over 360 min. For a solution of alcohol, three drinks resulted in pro-oxidant plasma on average, whereas while one drink did not significantly affect the plasma oxidative status. A preliminary experiment in which two volunteers showed a significantly increased time to metabolize ethanol after ingestion resulted in elevated antioxidant activity in plasma for lager beer and red wine.
One drink of red wine, beer, or stout provided equivalent increases in plasma antioxidant activity. Three drinks of red wine, beer, or stout provided equivalent increases in plasma pro-oxidant activity. This may explain, at least in part, the decreased risk of cataract and atherosclerosis from daily consumption of one drink of different types of alcoholic beverages as well as the increased risk from daily consumption of three drinks of alcoholic beverages. The plasma pro-oxidant activity appears to be due to ethanol metabolism, whereas the antioxidant activity may be due to the absorption of polyphenols in the beverages.
将饮用一杯或三杯不同饮料后空腹血浆的氧化状态与已知呈J形流行病学风险曲线相关联。
设计、干预措施及主要观察指标：比较红酒、贮藏啤酒、烈性黑啤酒（含酒精和不含酒精）的抗氧化活性以及酒精水溶液，观察人类志愿者饮用一杯或三杯含有等量酒精的典型饮料（不含酒精的烈性黑啤酒用作烈性黑啤酒的对照）后血浆抗氧化或促氧化活性。
饮用一杯红酒、贮藏啤酒或烈性黑啤酒（酒精度5%体积比，以及不含酒精的）显著提高了从志愿者处采集的血浆样本在240分钟内的平均抗氧化活性。饮用三杯红酒、贮藏啤酒或烈性黑啤酒（酒精度5%体积比，以及不含酒精的）显著提高了从志愿者处采集的血浆样本在360分钟内的平均促氧化活性。对于酒精溶液，饮用三杯平均导致血浆产生促氧化作用，而饮用一杯对血浆氧化状态无显著影响。一项初步实验中，两名志愿者摄入乙醇后代谢时间显著延长，结果贮藏啤酒和红酒的血浆抗氧化活性升高。
饮用一杯红酒、啤酒或烈性黑啤酒使血浆抗氧化活性有同等程度的增加。饮用三杯红酒、啤酒或烈性黑啤酒使血浆促氧化活性有同等程度的增加。这可能至少部分解释了每日饮用一杯不同类型酒精饮料可降低患白内障和动脉粥样硬化风险，而每日饮用三杯酒精饮料则风险增加的原因。血浆促氧化活性似乎归因于乙醇代谢，而抗氧化活性可能归因于饮料中多酚的吸收。
