Jordan Kate W, Nordenstam Johan, Lauwers Gregory Y, Rothenberger David A, Alavi Karim, Garwood Michael, Cheng Leo L
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Dis Colon Rectum. 2009 Mar;52(3):520-5. doi: 10.1007/DCR.0b013e31819c9a2c.
This study was designed to test whether metabolic characterization of intact, unaltered human rectal adenocarcinoma specimens is possible using the high-resolution magic angle spinning proton (1H) magnetic resonance spectroscopy technique.
The study included 23 specimens from five patients referred for ultrasonographic staging of suspected rectal cancer. Multiple biopsies of macroscopically malignant rectal tumors and benign rectal mucosa were obtained from each patient for a total of 14 malignant and 9 benign samples. Unaltered tissue samples were spectroscopically analyzed. Metabolic profiles were established from the spectroscopy data and correlated with histopathologic findings.
Metabolomic profiles represented by principle components of metabolites measured from spectra differentiated between malignant and benign samples and correlated with the volume percent of cancer (P = 0.0065 and P = 0.02, respectively) and benign epithelium (P = 0.0051 and P = 0.0255, respectively), and with volume percent of stroma, and inflammation.
Magnetic resonance spectroscopy of rectal biopsies has the ability to metabolically characterize samples and differentiate between pathological features of interest. Future studies should determine its utility in in vivo applications for non-invasive pathologic evaluations of suspicious rectal lesions.
本研究旨在测试使用高分辨率魔角旋转质子（1H）磁共振波谱技术对完整、未改变的人类直肠腺癌标本进行代谢特征分析是否可行。
该研究纳入了5例因疑似直肠癌接受超声分期的患者的23个标本。从每位患者的宏观恶性直肠肿瘤和良性直肠黏膜获取多个活检样本，共获得14个恶性样本和9个良性样本。对未改变的组织样本进行波谱分析。根据波谱数据建立代谢谱，并与组织病理学结果相关联。
由光谱测量的代谢物主成分代表的代谢组学谱在恶性和良性样本之间存在差异，并与癌组织的体积百分比（分别为P = 0.0065和P = 0.02）、良性上皮组织的体积百分比（分别为P = 0.0051和P = 0.0255）以及间质和炎症的体积百分比相关。
直肠活检的磁共振波谱能够对样本进行代谢特征分析，并区分感兴趣的病理特征。未来的研究应确定其在体内应用中对可疑直肠病变进行非侵入性病理评估的效用。
