Osiewacz H D, Ridder R
Deutsches Krebsforschungszentrum, Projektgruppe: Molekularbiologie der Alterungsprozesse, Heidelberg, Federal Republic of Germany.
Curr Genet. 1991 Jul;20(1-2):151-5. doi: 10.1007/BF00312778.
The gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) has been isolated from a genomic library of the filamentous fungus Curvularia lunata. The coding region of this gene consists of 1014 nucleotides and is interrupted by four introns. The gpd gene product shows a high degree of sequence identity with the corresponding proteins of various species belonging to both taxonomically related (e.g., Aspergillus nidulans), as well as more divergent, taxa. Using contour-clamped homogeneous electric field (CHEF) gel electrophoresis eight distinct chromosomal bands have been resolved, with two bands migrating as doublets and one as a triplet. Thus, the total number of chromosomes of C. lunata appears to be 12. The size of the chromosomes ranges from about 1.4 Mb to 4.0 Mb allowing an estimation of the genome to be approximately 29.7 Mb. By hybridization of fractionated chromosomes the gpd gene and the rDNA locus have been localized on individual chromosomes.
已从丝状真菌新月弯孢霉的基因组文库中分离出编码3-磷酸甘油醛脱氢酶（gpd）的基因。该基因的编码区由1014个核苷酸组成，并被四个内含子中断。gpd基因产物与分类学上相关的各种物种（如构巢曲霉）以及差异更大的分类群的相应蛋白质具有高度的序列同一性。使用轮廓夹钳均匀电场（CHEF）凝胶电泳解析出八条不同的染色体带，其中两条带以双峰形式迁移，一条以三峰形式迁移。因此，新月弯孢霉的染色体总数似乎为12条。染色体大小范围约为1.4 Mb至4.0 Mb，由此估计基因组约为29.7 Mb。通过分级染色体杂交，已将gpd基因和rDNA基因座定位到单个染色体上。
