Tang Xu-Dong, Xiao Qiang, Ma Xiu-Cui, Zhu Zeng-Rong, Zhang Chuan-Xi
Institute of Insect Science, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, China.
Virus Genes. 2009 Jun;38(3):495-506. doi: 10.1007/s11262-009-0355-0. Epub 2009 Apr 7.
Euproctis pseudoconspersa NPV (EupsNPV) is pathogenic to the tea tussock (E. pseudoconspersa), one of the major pests of tea bushes in East Asia, and has been used to control the pest. Electron microscope observation showed there were two modes for the virions embedded in each polyhedron, single-nucleocapsid and double-nucleocapsid. The EupsNPV genome contained 141,291 bp and had a G + C content of 40.4%. Of 139 potential ORFs predicted from the sequence, 126 had a homology in other baculoviruses; 13 were unique to EupsNPV. Four homologous repeat sequences (hrs) were present in the EupsNPV genome and the repeat sequences were different between these hrs. Three ORFs were identified to contain two homologues in the EupsNPV genome, including bro, p26 and dbp. Gene parity plots, percent identities of gene homologues and phylogenetic analysis all suggested that EupsNPV is most closely related to EcobNPV in Group II NPV, although its genomic organization was highly distinct.
茶毒蛾核型多角体病毒（EupsNPV）对茶毒蛾（E. pseudoconspersa）具有致病性，茶毒蛾是东亚茶树的主要害虫之一，该病毒已被用于防治这种害虫。电子显微镜观察显示，每个多面体中嵌入的病毒粒子有两种模式，即单核衣壳和双核衣壳。茶毒蛾核型多角体病毒基因组包含141,291个碱基对，G + C含量为40.4%。从该序列预测的139个潜在开放阅读框中，126个在其他杆状病毒中具有同源性；13个是茶毒蛾核型多角体病毒所特有的。茶毒蛾核型多角体病毒基因组中存在四个同源重复序列（hrs），这些重复序列在这些hrs之间有所不同。在茶毒蛾核型多角体病毒基因组中鉴定出三个开放阅读框包含两个同源物，包括bro、p26和dbp。基因同源性图、基因同源物的百分比同一性和系统发育分析均表明，茶毒蛾核型多角体病毒与II组核型多角体病毒中的EcobNPV关系最为密切，尽管其基因组组织高度不同。
