Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702, USA.
Genome Med. 2009 Jan 20;1(1):5. doi: 10.1186/gm5.
Metabolic profiling using mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) is integral to the rapidly expanding field of metabolomics, which is making progress in toxicology, plant science and various diseases, including cancer. In the area of oncology and metabolic phenotyping, researchers have probed the known changes in malignant cellular pathways using new experimental techniques to gain more insights, and others are exploiting these same cellular pathways for therapeutic drug targets and for novel cancer biomarkers, with the ultimate goal of translation to the clinic. Here, we discuss the challenges and opportunities in metabolic phenotyping for discovering novel cancer biomarkers, and we assess the clinical applicability of MS and NMR.
代谢组学是利用质谱（MS）和核磁共振波谱（NMR）进行代谢物分析的一门学科，它是毒理学、植物科学和各种疾病（包括癌症）领域迅速发展的重要组成部分。在肿瘤学和代谢表型领域，研究人员利用新的实验技术深入研究了恶性细胞通路的已知变化，以获得更多的见解，还有一些研究人员正在利用这些相同的细胞通路寻找治疗药物靶点和新型癌症生物标志物，最终目标是将其转化为临床应用。在这里，我们讨论了代谢表型学在发现新型癌症生物标志物方面所面临的挑战和机遇，并评估了 MS 和 NMR 的临床适用性。
