Bergamini F, Ferroni P
Boll Ist Sieroter Milan. 1977 Mar 31;56(1):15-21.
Titrations of complement fixing antibody (c.f.a.) were done in paired sera of 127 women at delivery and cord blood of their babies using two different cytomegalovirus strains both of which had undergone many passages in WI-38 cells, With High strain 74% of the mother and 41% of the babies were negative at the serum dilution 1:4. With Towne strain the incidence of negative was 5% both among the mother and the newborns. Worth of mention is the study of correlation of the titers in the matched pairs of sera according to the strain. With High strain 23 of the babies had c.f.a. titers significantly higher than their mothers and 14 of them were from negative women. This situation could be suggestive of an active placental transfer of IgG globulins. No such disparities were found using Towne strain as antigen. 48 pairs of sera were then tested with both strains by indirect hemagglutination, indirect immunofluorescence and gel precipitin test. Results are discussed in relation to the possible differences in the antigenic composition of the two cytomegalovirus strains.
使用两种不同的巨细胞病毒株（这两种病毒株均在WI-38细胞中传代多次），对127名产妇分娩时的配对血清及其婴儿的脐带血进行补体结合抗体（c.f.a.）滴定。对于高毒株，在血清稀释度为1:4时，74%的母亲和41%的婴儿呈阴性。对于汤氏毒株，母亲和新生儿中的阴性发生率均为5%。值得一提的是，根据毒株对配对血清中的滴度进行相关性研究。对于高毒株，23名婴儿的c.f.a.滴度明显高于其母亲，其中14名婴儿的母亲为阴性。这种情况可能提示IgG球蛋白存在活跃的胎盘转运。使用汤氏毒株作为抗原未发现此类差异。然后用间接血凝、间接免疫荧光和凝胶沉淀试验对48对血清进行两种毒株的检测。针对两种巨细胞病毒株抗原组成可能存在的差异对结果进行了讨论。
