Mieno M, Isaka Y, Kimura K, Matsumoto M, Etani H, Uehara A, Hashikawa K, Hata R, Moriwaki H, Ashida K
Division of Nuclear Medicine, Osaka University Medical School.
Kaku Igaku. 1990 May;27(5):443-9.
We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 10(6)/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 microM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 10(6)/microliters platelet concentration.
我们研究了各种铟 - 111水溶性螯合物和孵育介质对血小板标记效率及体外血小板聚集性的影响。在酸性枸橼酸盐葡萄糖 - 生理盐水（ACD - 盐水）中，硫酸铟 - 111、铟 - 111托酚酮和铟 - 111 - MPO（2 - 巯基吡啶 - N - 氧化物）可实现血小板的高标记效率。在酸性枸橼酸盐葡萄糖 - 血浆（ACD - 血浆）中血小板浓度为4.8×10⁶/mm³的条件下，硫酸铟 - 111 - 辛可宁的标记效率低且标记不一致，而铟 - 111托酚酮和铟 - 111 - MPO具有高标记效率。当在无血浆的情况下标记血小板时，体外血小板聚集性（ADP 2微摩尔）降低。然而，用三种不同螯合物标记的铟 - 111血小板之间的血小板聚集性没有显著差异。总之，为了在保持较高标记效率的同时维持血小板的聚集活性，在血小板浓度为4.8×10⁶/微升的血浆中使用MPO或托酚酮螯合物可获得最佳结果。
