Swarnakar Snehasikta, Paul Sumit
Department of Physiology, Drug Development Diagnostics and Biotechnology Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India.
Indian J Biochem Biophys. 2009 Feb;46(1):59-65.
Curcumin, a polyphenol derived from turmeric (Curcuma longa) possesses diverse pharmacological properties including antioxidant, anti-inflammatory and antiproliferative activities. Endometriosis is a gyneocological disorder characterized by growth of endometrial tissues outside uterus that involves aberrant matrix remodeling. In this study the effect of curcumin was studied on surgically developed endometriosis in mice. Endometriosis with varying severity was developed in mice by peritoneal implantation of uterine fragments. The changes in matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloprotease (TIMP)-1 were investigated in endometriotic tissues following curcumin pre- and posttreatment. Results showed that MMP-9 activity increased gradually in endometriotic tissues with severity and curcumin treatment reversed the MMP-9 activity near to control value. Curcumin administered either post- or pre-endometriosis arrested endometriosis in a dose-dependent manner. It inhibited both MMP-9 activity and its expression at the level of secretion, during regression of endometriotic lesion. In addition, the attenuated activity of MMP-9 was associated with decreased expression of tumor necrosis factor-alpha (TNF-alpha) during healing, suggesting the anti-inflammatory property of curcumin. Moreover, curcumin pretreatment prevented lipid peroxidation and protein oxidation in endometriotic tissues. We reported here for the first time the anti-endometriotic property of curcumin via MMP-9 dependent pathway that may lead to new therapeutic intervention.
姜黄素是一种从姜黄（姜黄属植物）中提取的多酚，具有多种药理特性，包括抗氧化、抗炎和抗增殖活性。子宫内膜异位症是一种妇科疾病，其特征是子宫内膜组织在子宫外生长，涉及异常的基质重塑。在本研究中，研究了姜黄素对小鼠手术诱导的子宫内膜异位症的影响。通过腹腔植入子宫碎片在小鼠中诱导出不同严重程度的子宫内膜异位症。在姜黄素治疗前后，研究了子宫内膜异位组织中基质金属蛋白酶（MMP）-9和金属蛋白酶组织抑制剂（TIMP）-1的变化。结果表明，MMP-9活性在子宫内膜异位组织中随着严重程度逐渐增加，而姜黄素治疗使MMP-9活性恢复到接近对照值。在子宫内膜异位症发生后或之前给予姜黄素均以剂量依赖的方式抑制子宫内膜异位症。在子宫内膜异位病变消退过程中，它在分泌水平上抑制MMP-9活性及其表达。此外，在愈合过程中，MMP-9活性的减弱与肿瘤坏死因子-α（TNF-α）表达的降低有关，这表明姜黄素具有抗炎特性。此外，姜黄素预处理可防止子宫内膜异位组织中的脂质过氧化和蛋白质氧化。我们首次在此报道姜黄素通过MMP-9依赖途径的抗子宫内膜异位特性，这可能导致新的治疗干预措施。
