Bromberg K, Tannis G, Steiner P
Department of Medicine, SUNY Health Science Center, Brooklyn 11203.
Infect Immun. 1991 Dec;59(12):4715-9. doi: 10.1128/iai.59.12.4715-4719.1991.
In humans, infection with Bordetella pertussis is considered to be localized to an epithelial surface. However, an intracellular state in cultured cells and in the macrophages of infected animals has been shown. By using indirect immunofluorescence with a monoclonal antibody, it was found that 3 of 20 bronchoalveolar lavage specimens from children with human immunodeficiency virus infection had B. pertussis associated with pulmonary alveolar macrophages. None of the cultures from the patients grew B. pertussis. The B. pertussis appeared to be intracellular.
在人类中，百日咳博德特氏菌感染被认为局限于上皮表面。然而，已证实在培养细胞以及受感染动物的巨噬细胞中存在细胞内状态。通过使用单克隆抗体进行间接免疫荧光检测，发现20份来自感染人类免疫缺陷病毒儿童的支气管肺泡灌洗标本中有3份的肺泡巨噬细胞与百日咳博德特氏菌相关。患者的培养物均未培养出百日咳博德特氏菌。百日咳博德特氏菌似乎处于细胞内。
