Makrigiannakis A, Karamouti M, Petsas G, Makris N, Nikas G, Antsaklis A
In Vitro Fertilization Unit, Department of Obstetrics and Gynaecology, Medical School, University of Crete, Heraklion, 71003, Greece.
Histochem Cell Biol. 2009 Aug;132(2):159-67. doi: 10.1007/s00418-009-0593-1. Epub 2009 Apr 23.
Pinopodes represent the morphological and integrins, the biomolecular markers of endometrial receptivity. We studied using scanning electron microscopy, the expression of pinopodes on tubal samples and their corresponding endometria, from 21 women of reproductive age (7 from proliferative phase, 7 from day LH +5 and 7 from day LH +7). In addition, we examined the immunohistochemical staining of integrins alpha v beta 3, alpha v beta 5 and their ligands, fibronectin (FN) and osteopontin (OPN) in the same tubal epithelium samples. Pinopodes were detected on the tubal epithelium exclusively during day LH +7, coincident with their formation in the endometrium and synchronous to alpha v beta 3 sharp increase in the oviduct epithelium, suggesting a regulation similar to the endometrium. In contrast, alpha v beta 5, FN and OPN remained unchanged during the cycle. These results show for the first time the formation of pinopodes in the tubal epithelium at the time of endometrial receptivity and correlate it with the upregulation of the intact dimmer alpha v beta 3 in the tubes.
胞饮突代表子宫内膜容受性的形态学和整合素等生物分子标志物。我们使用扫描电子显微镜研究了21名育龄妇女（7名处于增殖期，7名处于LH +5天，7名处于LH +7天）输卵管样本及其相应子宫内膜上胞饮突的表达。此外，我们检测了相同输卵管上皮样本中整合素αvβ3、αvβ5及其配体纤连蛋白（FN）和骨桥蛋白（OPN）的免疫组织化学染色。仅在LH +7天在输卵管上皮检测到胞饮突，这与它们在子宫内膜中的形成一致，并且与输卵管上皮中αvβ3的急剧增加同步，表明存在与子宫内膜类似的调节。相比之下，αvβ5、FN和OPN在整个周期中保持不变。这些结果首次显示在子宫内膜容受性时输卵管上皮中胞饮突的形成，并将其与输卵管中完整二聚体αvβ3的上调相关联。
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