Institute of Bioinformatics and Biotechnology, India.
Evid Based Complement Alternat Med. 2011;2011:561625. doi: 10.1093/ecam/nep033. Epub 2011 Jun 18.
Diabetes mellitus is a metabolic syndrome characterized by an increase in the blood glucose level. Treatment of diabetes is complicated due to multifactorial nature of the disease. Azadirachta indica Adr. Juss and Bougainvillea spectabilis are reported to have medicinal values including antidiabetic properties. In the present study using invivo diabetic murine model, A. indica and B. spectabilis chloroform, methanolic and aqueous extracts were investigated for the biochemical parameters important for controlling diabetes. It was found that A. indica chloroform extract and B. spectabilis aqueous, methanolic extracts showed a good oral glucose tolerance and significantly reduced the intestinal glucosidase activity. Interestingly, A. indica chloroform and B. spectabilis aqueous extracts showed significant increase in glucose-6-phosphate dehydrogenase activity and hepatic, skeletal muscle glycogen content after 21 days of treatment. In immunohistochemical analysis, we observed a regeneration of insulin-producing cells and corresponding increase in the plasma insulin and c-peptide levels with the treatment of A. indica chloroform and B. spectabilis aqueous, methanolic extracts. Analyzing the results, it is clear that A. indica chloroform and B. spectabilis aqueous extracts are good candidates for developing new neutraceuticals treatment for diabetes.
糖尿病是一种代谢综合征，其特征是血糖水平升高。由于该疾病的多因素性质，糖尿病的治疗较为复杂。已报道印楝（Azadirachta indica Adr. Juss）和使君子（Bougainvillea spectabilis）具有药用价值，包括抗糖尿病特性。在本研究中，使用体内糖尿病小鼠模型，研究了印楝和使君子的氯仿、甲醇和水提取物对控制糖尿病的重要生化参数的影响。结果发现，印楝的氯仿提取物和使君子的水提物、甲醇提取物具有良好的口服葡萄糖耐量，并显著降低了肠道葡萄糖苷酶活性。有趣的是，经过 21 天的治疗后，印楝的氯仿提取物和使君子的水提取物显著增加了葡萄糖-6-磷酸脱氢酶活性以及肝、骨骼肌中的糖原含量。在免疫组织化学分析中，我们观察到胰岛素产生细胞的再生，并且随着印楝的氯仿提取物和使君子的水提物、甲醇提取物的治疗，血浆胰岛素和 C 肽水平相应增加。通过分析结果，显然印楝的氯仿提取物和使君子的水提取物是开发用于治疗糖尿病的新型营养保健品的良好候选物。
