一种用于检测艰难梭菌毒素的超灵敏快速免疫细胞毒性测定法。
An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins.
作者信息
He Xiangyun, Wang Jufang, Steele Jennifer, Sun Xingmin, Nie Weijia, Tzipori Saul, Feng Hanping
机构信息
Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.
出版信息
J Microbiol Methods. 2009 Jul;78(1):97-100. doi: 10.1016/j.mimet.2009.04.007. Epub 2009 Apr 23.
Abstract
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.
摘要
我们描述了一种用于检测艰难梭菌毒素A和B的新型超灵敏细胞免疫细胞毒性测定法。该测定法操作简单，周转时间约为3小时。它在检测浓度低于1 pg/ml的TcdA时特别灵敏。使用该测定法，我们能够在实验感染仔猪的粪便和血清样本中检测到艰难梭菌毒素的存在。
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1
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Infect Immun. 2009 Jun;77(6):2294-303. doi: 10.1128/IAI.01577-08. Epub 2009 Mar 23.
2
Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium.
BMC Microbiol. 2008 Nov 6;8:192. doi: 10.1186/1471-2180-8-192.
3
Diagnosis of Clostridium difficile infection by toxin detection kits: a systematic review.
Lancet Infect Dis. 2008 Dec;8(12):777-84. doi: 10.1016/S1473-3099(08)70233-0. Epub 2008 Nov 1.
4
Clostridium difficile--more difficult than ever.
N Engl J Med. 2008 Oct 30;359(18):1932-40. doi: 10.1056/NEJMra0707500.
5
Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea.
Clin Infect Dis. 2007 Nov 1;45(9):1152-60. doi: 10.1086/522185. Epub 2007 Sep 25.
6
Narrative review: the new epidemic of Clostridium difficile-associated enteric disease.
Ann Intern Med. 2006 Nov 21;145(10):758-64. doi: 10.7326/0003-4819-145-10-200611210-00008.
7
Identification of Clostridium difficile toxin B cardiotoxicity using a zebrafish embryo model of intoxication.
Proc Natl Acad Sci U S A. 2006 Sep 19;103(38):14176-81. doi: 10.1073/pnas.0604725103. Epub 2006 Sep 11.
8
Label-free, real-time monitoring of IgE-mediated mast cell activation on microelectronic cell sensor arrays.
J Immunol Methods. 2004 Sep;292(1-2):195-205. doi: 10.1016/j.jim.2004.06.022.
9
Clostridium difficile testing: after 20 years, still challenging.
J Clin Microbiol. 2003 Feb;41(2):531-4. doi: 10.1128/JCM.41.2.531-534.2003.
10
Clinical practice. Antibiotic-associated diarrhea.
N Engl J Med. 2002 Jan 31;346(5):334-9. doi: 10.1056/NEJMcp011603.
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