Touati A, Benard A, Hassen A Ben, Bébéar C M, Pereyre S
Service des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, Tunisia.
J Clin Microbiol. 2009 Jul;47(7):2269-71. doi: 10.1128/JCM.00326-09. Epub 2009 Apr 29.
The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.
与一种内部实时聚合酶链反应(PCR)相比,评估了五种用于检测呼吸道标本中肺炎支原体的商业TaqMan实时PCR检测方法的性能。所有试剂盒均能得出快速且特异的结果,并通过使用内部对照进行了验证。Nanogen试剂盒显示出最佳的临床敏感性。