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Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.对转录间隔区2的20个核苷酸进行焦磷酸测序分析可区分近平滑念珠菌、间型念珠菌和正平滑念珠菌。
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2
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本文引用的文献

1
Geographic distribution and antifungal susceptibility of the newly described species Candida orthopsilosis and Candida metapsilosis in comparison to the closely related species Candida parapsilosis.新描述的近平滑念珠菌和季也蒙念珠菌与密切相关的副平滑念珠菌相比的地理分布及抗真菌药敏性
J Clin Microbiol. 2008 Aug;46(8):2659-64. doi: 10.1128/JCM.00803-08. Epub 2008 Jun 18.
2
DNA pyrosequencing-based identification of pathogenic Candida species by using the internal transcribed spacer 2 region.基于焦磷酸测序技术，利用转录间隔区2区域对致病性念珠菌进行菌种鉴定。
Arch Pathol Lab Med. 2008 Apr;132(4):667-74. doi: 10.5858/2008-132-667-DPIOPC.
3
Prevalence and susceptibility profile of Candida metapsilosis and Candida orthopsilosis: results from population-based surveillance of candidemia in Spain.近平滑念珠菌和正平滑念珠菌的患病率及易感性概况：西班牙念珠菌血症人群监测结果
Antimicrob Agents Chemother. 2008 Apr;52(4):1506-9. doi: 10.1128/AAC.01595-07. Epub 2008 Feb 19.
4
Candida nivariensis, an emerging pathogenic fungus with multidrug resistance to antifungal agents.尼氏假丝酵母，一种对抗真菌药物具有多重耐药性的新兴致病真菌。
J Clin Microbiol. 2008 Mar;46(3):933-8. doi: 10.1128/JCM.02116-07. Epub 2008 Jan 16.
5
Molecular identification of pathogenic fungi.致病真菌的分子鉴定
J Antimicrob Chemother. 2008 Jan;61 Suppl 1:i7-12. doi: 10.1093/jac/dkm425.
6
Genotyping of Candida orthopsilosis clinical isolates by amplification fragment length polymorphism reveals genetic diversity among independent isolates and strain maintenance within patients.通过扩增片段长度多态性对近平滑念珠菌临床分离株进行基因分型，揭示了独立分离株之间的遗传多样性以及患者体内的菌株维持情况。
J Clin Microbiol. 2007 May;45(5):1455-62. doi: 10.1128/JCM.00243-07. Epub 2007 Feb 28.
7
Ultra-rapid preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter paper technology - a reusable DNA archiving system.使用沃特曼FTA滤纸技术从酵母和霉菌分离物中超快速制备全基因组DNA——一种可重复使用的DNA存档系统。
Med Mycol. 2006 Aug;44(5):389-98. doi: 10.1080/13693780600564613.
8
Epidemiology of Candida albicans infections and role of non-Candida-albicans yeasts.白色念珠菌感染的流行病学及非白色念珠菌酵母的作用。
Curr Drug Targets. 2006 Apr;7(4):495-504. doi: 10.2174/138945006776359421.
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Epidemiology of candidemia.念珠菌血症的流行病学
Rev Iberoam Micol. 2000 Sep;17(3):73-81.
10
Detection and identification of Candida species associated with Candida vaginitis by real-time PCR and pyrosequencing.通过实时聚合酶链反应和焦磷酸测序法检测与鉴定念珠菌性阴道炎相关的念珠菌属菌种
Mol Cell Probes. 2005 Apr;19(2):145-52. doi: 10.1016/j.mcp.2004.10.004. Epub 2005 Jan 20.

对转录间隔区2的20个核苷酸进行焦磷酸测序分析可区分近平滑念珠菌、间型念珠菌和正平滑念珠菌。

Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.

作者信息

Borman Andrew M, Linton Christopher J, Oliver Debra, Palmer Michael D, Szekely Adrien, Odds Frank C, Johnson Elizabeth M

机构信息

Mycology Reference Laboratory, Health Protection Agency, Bristol, United Kingdom.

出版信息

J Clin Microbiol. 2009 Jul;47(7):2307-10. doi: 10.1128/JCM.00240-09. Epub 2009 Apr 29.

DOI:10.1128/JCM.00240-09
PMID:19403763
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2708508/
Abstract

Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

摘要

最近，通过基因异质性近平滑念珠菌复合体中几个基因之间一致的DNA序列差异，鉴定出了两个新的隐匿姐妹种，即正平滑念珠菌和变平滑念珠菌。在此，我们展示的数据表明，对转录间隔区2的20个核苷酸进行焦磷酸测序分析能够快速且可靠地区分这三种密切相关的念珠菌。