Jiang Z D, Nelson A C, Mathewson J J, Ericsson C D, DuPont H L
Center for Infectious Diseases, University of Texas Medical School, Houston.
J Infect Dis. 1991 Nov;164(5):979-82. doi: 10.1093/infdis/164.5.979.
Intestinal secretory IgA (sIgA) response or lack of response among adults in Mexico with diarrhea was used as an indicator of enteropathogenicity of Aeromonas species and Plesiomonas shigelloides. sIgA was extracted from stool specimens obtained at day of presentation and 5 days later. Total sIgA was standardized, and specific sIgA titer against the organism being shed by each patient was determined. Western blotting was used to determine which microbial antigens elicited an intestinal sIgA response. Of 12 subjects shedding Aeromonas sobria or Aeromonas hydrophila, 11 had a fourfold or greater sIgA titer rise against the infecting strain. Western blotting showed that somatic lipopolysaccharides were the immunodominant antigens. No sIgA titer rises were detected among two patients shedding Aeromonas caviae or in 14 shedding P. shigelloides. This study provides further evidence of the significance of A. sobria and A. hydrophila as pathogens in acute diarrhea but raises additional questions about the role of P. shigelloides, at least in US adults with travelers' diarrhea.
在墨西哥，将患有腹泻的成年人肠道分泌型IgA（sIgA）反应或无反应用作嗜水气单胞菌属和类志贺邻单胞菌肠道致病性的指标。sIgA从就诊当天及5天后采集的粪便标本中提取。对总sIgA进行标准化，并测定针对每位患者排出的病原体的特异性sIgA滴度。采用蛋白质印迹法确定哪些微生物抗原可引发肠道sIgA反应。在12名排出温和气单胞菌或嗜水气单胞菌的受试者中，11名针对感染菌株的sIgA滴度升高了四倍或更高。蛋白质印迹法显示，体细胞脂多糖是免疫显性抗原。在两名排出豚鼠气单胞菌的患者或14名排出类志贺邻单胞菌的患者中，未检测到sIgA滴度升高。本研究进一步证明了温和气单胞菌和嗜水气单胞菌作为急性腹泻病原体的重要性，但也提出了关于类志贺邻单胞菌作用的更多问题，至少在美国患有旅行者腹泻的成年人中是这样。
