Zhao Guo-Zhen, Li Jie, Qin Sheng, Zhang Yu-Qin, Zhu Wen-Yong, Jiang Cheng-Lin, Xu Li-Hua, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2383-7. doi: 10.1099/ijs.0.010256-0. Epub 2009 Jul 20.
In this study, strain YIM 65004(T), isolated from roots of Polyspora axillaris, was shown to represent a novel species of the genus Micrococcus by means of a polyphasic approach. Chemotaxonomic data gathered for peptidoglycan type, menaquinones, phospholipids and fatty acids strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine, glycine and aspartic acid, the predominant menaquinones were MK-8(H(2)) (66.97 %) and MK-7(H(2)) (23.26 %), the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid, and the major cellular fatty acids were anteiso-C(15 : 0) (61.98 %), iso-C(16 : 0) (14.25 %) and iso-C(15 : 0) (13.04 %). The G+C content of the genomic DNA was 71.7 mol%. A number of physiological features were found that clearly distinguished strain YIM 65004(T) from recognized Micrococcus species. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. Based on the above data, a novel species of the genus Micrococcus, Micrococcus yunnanensis sp. nov., is proposed, with the type strain YIM 65004(T) (=CCTCC AA 208060(T)=DSM 21948(T)).
在本研究中，通过多相分类法表明，从腋花荷根部分离得到的菌株YIM 65004(T)代表了微球菌属的一个新物种。收集到的关于肽聚糖类型、甲基萘醌、磷脂和脂肪酸的化学分类数据有力地支持了该菌株在微球菌属内的分类：细胞壁肽聚糖含有赖氨酸、谷氨酸、丙氨酸、甘氨酸和天冬氨酸，主要甲基萘醌为MK-8(H(2))（66.97%）和MK-7(H(2))（23.26%），磷脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种未知的茚三酮阴性磷脂，主要细胞脂肪酸为anteiso-C(15 : 0)（61.98%）、iso-C(16 : 0)（14.25%）和iso-C(15 : 0)（13.04%）。基因组DNA的G+C含量为71.7 mol%。发现了许多生理特征，这些特征明显将菌株YIM 65004(T)与已认可的微球菌物种区分开来。DNA-DNA杂交研究表明，该新菌株代表一个独立的基因组物种。基于上述数据，提出了微球菌属的一个新物种，即云南微球菌（Micrococcus yunnanensis sp. nov.），模式菌株为YIM 65004(T)（=CCTCC AA 208060(T)=DSM 21948(T)）。
