Yao C Y, Wang J, Dong D, Qian F G, Xie J, Pan S L
School of Pharmacy, Fudan University, Shanghai, China.
Phytomedicine. 2009 Sep;16(9):823-9. doi: 10.1016/j.phymed.2009.02.008. Epub 2009 May 15.
In the present work, we studied the effect of laetispicine, an amide alkaloid isolated from the stems of Piper laetispicum (Piperaceae), in forced swimming, open field, acetic acid writhing and formalin tests in KM mice to assess antidepressant and antinociceptive effects. A significant and dose-dependent decrease in the immobility time, as evaluated by the forced swimming test, was observed after laetispicine administration (38.18, 39.79, 58.77 and 67.28% decreased at the doses of 5, 10, 20, 40mg/kg, respectively), suggesting an antidepressant effect. Furthermore, in the open field test, laetispicine at the given doses did not alter the number of crossings and rearing, as compared to controls. Results from writhing and formalin tests showed that laetispicine reduced the number of writhing in mice in a dose-dependent manner, attenuated the licking and spiting time of the injected paw in the first phase of formalin test. The antinociceptive effect of laetispicine was not affected by pre-treatment (i.p.) with naloxone (2mg/kg). In conclusion, we showed that laetispicine possessed significant antidepressant and antinociceptive properties, making this drug potentially useful in depression and pain.
在本研究中，我们研究了从胡椒科植物光轴胡椒茎中分离得到的酰胺生物碱光轴胡椒碱在KM小鼠的强迫游泳、旷场、醋酸扭体和福尔马林试验中的作用，以评估其抗抑郁和抗伤害感受作用。给予光轴胡椒碱后，通过强迫游泳试验评估，不动时间显著且呈剂量依赖性降低（在5、10、20、40mg/kg剂量下分别降低38.18%、39.79%、58.77%和67.28%），表明有抗抑郁作用。此外，在旷场试验中，与对照组相比，给定剂量的光轴胡椒碱并未改变穿越次数和直立次数。扭体试验和福尔马林试验结果表明，光轴胡椒碱以剂量依赖性方式减少小鼠的扭体次数，在福尔马林试验的第一阶段减轻注射爪的舔舐和吐口水时间。光轴胡椒碱的抗伤害感受作用不受纳洛酮（2mg/kg，腹腔注射）预处理的影响。总之，我们表明光轴胡椒碱具有显著的抗抑郁和抗伤害感受特性，使该药物在治疗抑郁症和疼痛方面具有潜在用途。
