Jalonen T, Isolauri E, Heyman M, Crain-Denoyelle A M, Sillanaukee P, Koivula T
Department of Clinical Sciences, University of Tampere, Finland.
Pediatr Res. 1991 Sep;30(3):290-3. doi: 10.1203/00006450-199109000-00019.
We studied absorption of the potentially allergenic protein beta-lactoglobulin during acute rotavirus diarrhea in infants and assessed the relationship of this macromolecular absorption with intestinal sugar permeability. After oral rehydration, 38 patients with acute gastroenteritis were given orally a 100-ml solution containing 4 g (11.7 mmol/L) of lactulose and 0.8 g (4.4 mmol/L) of mannitol, and their recovery rate as shown in urine passed during the subsequent 5 h was measured. A blood sample was taken 2 h after a milk feed for ELISA measurement of beta-lactoglobulin in circulating immune complexes. Twelve nondiarrhea patients were studied after an overnight fast as controls. Immune complexes containing beta-lactoglobulin were found in the serum of all, but the levels [median (range)] were significantly higher in patients with rotavirus diarrhea [686 (36-4352)] than in nondiarrhea patients [165 (0-2594)]; p = 0.007. The mean (95% confidence interval) lactulose/mannitol urinary recovery ratios were increased in patients with acute diarrhea [0.19 (0.10, 0.30)] compared to nondiarrhea patients [0.01 (0.005, 0.02)]; p = 0.0001. Thus, a significant correlation between beta-lactoglobulin absorption and sugar permeability was found; Spearman's rank correlation coefficient = 0.42, p = 0.004. This correlation was not, however, direct but was due to an inverse relationship between urinary recovery of mannitol and serum beta-lactoglobulin immune complexes. These results indicate that rotavirus gastroenteritis is associated with enhanced beta-lactoglobulin absorption and elevated lactulose/mannitol permeability test results, but these represent different phenomena.
我们研究了婴儿急性轮状病毒腹泻期间潜在致敏蛋白β-乳球蛋白的吸收情况，并评估了这种大分子吸收与肠道糖通透性之间的关系。口服补液后，给38例急性胃肠炎患者口服含4 g（11.7 mmol/L）乳果糖和0.8 g（4.4 mmol/L）甘露醇的100 ml溶液，并测量随后5小时尿液中的回收率。喂奶2小时后采集血样，用酶联免疫吸附测定法检测循环免疫复合物中的β-乳球蛋白。12例非腹泻患者空腹过夜后作为对照进行研究。所有患者血清中均发现含有β-乳球蛋白的免疫复合物，但轮状病毒腹泻患者的水平[中位数（范围）]显著高于非腹泻患者[686（36 - 4352）] 比[165（0 - 2594）]；p = 0.007。与非腹泻患者[0.01（0.005，0.02）]相比，急性腹泻患者的乳果糖/甘露醇尿回收率平均值（95%置信区间）升高[0.19（0.10，0.30）]；p = 0.0001。因此，发现β-乳球蛋白吸收与糖通透性之间存在显著相关性；Spearman等级相关系数 = 0.42，p = 0.004。然而，这种相关性并非直接相关，而是由于甘露醇尿回收率与血清β-乳球蛋白免疫复合物之间呈负相关。这些结果表明，轮状病毒胃肠炎与β-乳球蛋白吸收增强和乳果糖/甘露醇通透性试验结果升高有关，但这些代表了不同的现象。
