Crist Betsy L, Alekel D Lee, Ritland Laura M, Hanson Laura N, Genschel Ulrike, Reddy Manju B
Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011, USA.
J Womens Health (Larchmt). 2009 Jun;18(6):795-801. doi: 10.1089/jwh.2008.0988.
Centralized adiposity, insulin resistance, excess iron, and elevated oxidative stress place postmenopausal women at risk for atherosclerotic cardiovascular disease (CVD). The objective of this study was to determine the relationship among excess iron, oxidative stress, and centralized fat mass in healthy postmenopausal women.
The parent project recruited healthy women for a randomized, double-blind, clinical trial designed to examine the effect of soy isoflavones on bone. At baseline (n = 122), we measured three antioxidant enzymes, iron status indices (serum ferritin among others), oxidative stress indices (oxidized low-density lipoprotein [oxLDL], urinary isoprostanes [PGF(2alpha)], protein carbonyls, DNA damage), and waist, hip, and thigh fat mass using dual-energy x-ray absorptiometry (DXA). We calculated insulin resistance using the homeostasis model assessment (HOMA). Multiple regression analysis was used to determine the CVD risk factors that contributed to oxidative stress and centralized fat mass (waist + hip/thigh = AndGynFM ratio).
Almost 14% (p < 0.0005) of the variability in oxLDL was accounted for by AndGynFM ratio (6.1%, p < 0.0005), age (4.0%, p = 0.012), and serum iron (2.8%, p = 0.053). Similarly, 16% (p < 0.0001) of the variability in PGF(2alpha) was accounted for by the AndGynFM ratio (4.8%, p = 0.011), HOMA (3.9%, p = 0.021), and serum iron (2.7%, p = 0.054). We accounted for 33% (p </= 0.0001) of the variability in AndGynFM ratio by high-density lipoprotein cholesterol (HDL-C) (4.3%, p = 0.008), ferritin (4.9%, p = 0.005), HOMA (4.5%, p = 0.006), oxLDL (2.6%, p = 0.04), and PGF(2alpha) (3.0%, p = 0.025).
Our study suggests that reducing centralized fat mass and maintaining a favorable lipid profile, antioxidant status, and iron status all may be important in protecting postmenopausal women from atherosclerotic CVD.
中心性肥胖、胰岛素抵抗、铁过量及氧化应激增强使绝经后女性面临动脉粥样硬化性心血管疾病（CVD）风险。本研究的目的是确定健康绝经后女性体内铁过量、氧化应激与中心性脂肪量之间的关系。
母项目招募健康女性参与一项随机、双盲临床试验，旨在研究大豆异黄酮对骨骼的影响。在基线时（n = 122），我们测量了三种抗氧化酶、铁状态指标（包括血清铁蛋白等）、氧化应激指标（氧化型低密度脂蛋白[oxLDL]、尿中异前列腺素[PGF(2α)]、蛋白质羰基、DNA损伤），并使用双能X线吸收法（DXA）测量了腰围、臀围和大腿脂肪量。我们使用稳态模型评估（HOMA）计算胰岛素抵抗。采用多元回归分析来确定导致氧化应激和中心性脂肪量（腰围 + 臀围/大腿 = 雌雄脂肪量比[AndGynFM ratio]）的CVD危险因素。
AndGynFM比（6.1%，p < 0.0005）、年龄（4.0%，p = 0.012）和血清铁（2.8%，p = 0.053）占oxLDL变异性的近14%（p < 0.0005）。同样，AndGynFM比（4.8%，p = 0.011）、HOMA（3.9%，p = 0.021）和血清铁（2.7%，p = 0.054）占PGF(2α)变异性的16%（p < 0.0001）。高密度脂蛋白胆固醇（HDL-C）（4.3%，p = 0.008）、铁蛋白（4.9%，p = 0.005）、HOMA（4.5%，p = 0.006）、oxLDL（2.6%，p = 0.04）和PGF(2α)（3.0%，p = 0.025）占AndGynFM比变异性的33%（p ≤ 0.0001）。
我们的研究表明，减少中心性脂肪量并维持良好的血脂谱、抗氧化状态和铁状态，对于保护绝经后女性免受动脉粥样硬化性CVD可能都很重要。
