Moon Seo Hyun, Jang Yoon-Jeong, Han Myun Soo, Cho Myung-Haing
Department of Forensic DNA, National Forensic Service, Wonju 26460, Korea.
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
J Vet Sci. 2016 Sep 30;17(3):391-8. doi: 10.4142/jvs.2016.17.3.391.
Dogs have long shared close relationships with many humans. Due to the large number of dogs in human populations, they are often involved in crimes. Occasionally, canine biological evidence such as saliva, bloodstains and hairs can be found at crime scenes. Accordingly, canine DNA can be used as forensic evidence. The use of short tandem repeat (STR) loci from biological evidence is valuable for forensic investigations. In Korea, canine STR profiling-related crimes are being successfully analyzed, leading to diverse crimes such as animal cruelty, dog-attacks, murder, robbery, and missing and abandoned dogs being solved. However, the probability of random DNA profile matches cannot be analyzed because of a lack of canine STR data. Therefore, in this study, 10 STR loci were analyzed in 600 dogs in Korea (344 dogs belonging to 30 different purebreds and 256 crossbred dogs) to estimate canine forensic genetic parameters. Among purebred dogs, a separate statistical analysis was conducted for five major subgroups, 97 Maltese, 47 Poodles, 31 Shih Tzus, 32 Yorkshire Terriers, and 25 Pomeranians. Allele frequencies, expected (Hexp) and observed heterozygosity (Hobs), fixation index (F), probability of identity (P(ID)), probability of sibling identity (P(ID)sib) and probability of exclusion (PE) were then calculated. The Hexp values ranged from 0.901 (PEZ12) to 0.634 (FHC2079), while the P(ID)sib values were between 0.481 (FHC2079) and 0.304 (PEZ12) and the P(ID)sib was about 3.35 × 10(-)⁵ for the combination of all 10 loci. The results presented herein will strengthen the value of canine DNA to solving dog-related crimes.
长期以来，狗与许多人类建立了亲密关系。由于人类社会中狗的数量众多，它们常常与犯罪活动有所关联。偶尔，在犯罪现场能够发现狗的生物证据，如唾液、血迹和毛发等。因此，狗的DNA可作为法医证据使用。利用生物证据中的短串联重复序列（STR）位点对法医调查具有重要价值。在韩国，与犬类STR图谱分析相关的犯罪案件正得到成功分析，促使各类犯罪得以侦破，包括虐待动物、犬类袭击、谋杀、抢劫以及失踪和被遗弃犬类案件。然而，由于缺乏犬类STR数据，无法分析随机DNA图谱匹配的概率。所以，在本研究中，对韩国的600只狗（344只属于30个不同纯种犬种的狗和256只杂种狗）的10个STR位点进行了分析，以估算犬类法医遗传参数。在纯种犬中，对五个主要亚组进行了单独的统计分析，分别为97只马尔济斯犬、47只贵宾犬、31只西施犬、32只约克夏梗犬和25只博美犬。随后计算了等位基因频率、期望杂合度（Hexp）和观察杂合度（Hobs）、固定指数（F）、个体识别概率（P(ID)）、同胞识别概率（P(ID)sib）以及排除概率（PE）。Hexp值范围为0.901（PEZ12）至0.634（FHC2079），而P(ID)sib值在0.481（FHC2079）至0.304（PEZ12）之间，所有10个位点组合的P(ID)sib约为3.35×10⁻⁵。本文呈现的结果将增强犬类DNA在解决与狗相关犯罪中的价值。
