Chan Wen-Hsiung
Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, 200, Chung Pei Road, Chung Li 32023, Taiwan.
Reprod Toxicol. 2009 Jul;28(1):52-8. doi: 10.1016/j.reprotox.2009.03.014. Epub 2009 Apr 9.
Genistein (GNT), a natural isoflavone compound found in soy products, affects diverse cell functions, including proliferation, differentiation and cell death. An earlier study by our group showed that GNT has cytotoxic effects on mouse blastocysts and is associated with defects in their subsequent development in vitro. Here, we further investigate the effects of GNT on oocyte maturation, and subsequent pre- and postimplantation development, both in vitro and in vivo. GNT induced a significant reduction in the rate of oocyte maturation, fertilization, and in vitro embryo development. Treatment of oocytes with GNT during in vitro maturation (IVM) led to increased resorption of postimplantation embryos, and decreased placental and fetal weights. With the aid of an in vivo mouse model, we showed that consumption of drinking water containing GNT led to decreased oocyte maturation and in vitro fertilization, as well as early embryonic developmental injury. Moreover, our findings support a degree of selective inhibition of retinoic acid receptors in blastocysts treated with GNT during oocyte maturation. To our knowledge, this is the first study investigating the impact of GNT on maturation of mouse oocytes, fertilization, and sequential embryonic development.
金雀异黄素（GNT）是一种存在于豆制品中的天然异黄酮化合物，会影响多种细胞功能，包括增殖、分化和细胞死亡。我们团队早期的一项研究表明，GNT对小鼠囊胚具有细胞毒性作用，并与它们随后在体外发育的缺陷有关。在此，我们进一步研究GNT对卵母细胞成熟以及随后体外和体内植入前和植入后发育的影响。GNT导致卵母细胞成熟率、受精率和体外胚胎发育率显著降低。在体外成熟（IVM）过程中用GNT处理卵母细胞会导致植入后胚胎的吸收增加，胎盘和胎儿重量降低。借助体内小鼠模型，我们表明饮用含GNT的水会导致卵母细胞成熟和体外受精减少，以及早期胚胎发育损伤。此外，我们的研究结果支持在卵母细胞成熟过程中用GNT处理的囊胚中对维甲酸受体有一定程度的选择性抑制。据我们所知，这是第一项研究GNT对小鼠卵母细胞成熟、受精和连续胚胎发育影响的研究。
