Hong Guiyun, Jiang Shaotong, Yu Miao, Yang Ying, Li Feng, Xue Fangsen, Wei Zhaojun
Department of Biotechnology, Hefei University of Technology, Hefei 230009, China.
Acta Biochim Biophys Sin (Shanghai). 2009 Jun;41(6):446-55. doi: 10.1093/abbs/gmp030.
The complete mitochondrial genome (mitogenome) of Artogeia melete was determined as being composed of 15,140 bp, including 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and one control region. The gene order of A. melete mitogenome is typical of Lepidoptera and differs from the insect ancestral type in the location of trnM. The A. melete mitogenome has a total of 119 bp of intergenic spacer sequences spread over 10 regions, ranging in sizes between 1 and 48 bp. The nucleotide composition of the A. melete mitogenome is also biased toward A 1 T nucleotides (79.77%), which is higher than that of Ochrogaster lunifer (77.84%), but lower than nine other lepidopterans sequenced. The PCGs have typical mitochondrial start codons, except for cox1, which contains the unusual CGA. The cox1, cox2, nad2, and nad5 genes of the A. melete mitogenome have incomplete stop codons (T). The A. melete A 1 T-rich region contains some conserved structures that are similar to those found in other lepidopteran mitogenomes, including a structure combining the motif ATAGA, a 19-bp poly(T) stretch, a microsatellite (AT)n element, and a 9-bp poly(A) upstream trnM. The A. melete mitogenome contains a duplicated 36-bp repeat element, which consists of a 26- bp core sequence flanked by 10-bp perfectly inverted repeats.
美凤蝶的完整线粒体基因组(线粒体基因组)被确定为由15140个碱基对组成,包括13个蛋白质编码基因(PCGs)、2个rRNA基因、22个tRNA基因和一个控制区。美凤蝶线粒体基因组的基因排列顺序是鳞翅目典型的,并且在trnM的位置上与昆虫祖先类型不同。美凤蝶线粒体基因组共有119个碱基对的基因间隔序列,分布在10个区域,大小在1至48个碱基对之间。美凤蝶线粒体基因组的核苷酸组成也偏向于A和T核苷酸(79.77%),高于赭夜蛾(77.84%),但低于其他已测序的9种鳞翅目昆虫。除了cox1含有不寻常的CGA外,PCGs具有典型的线粒体起始密码子。美凤蝶线粒体基因组的cox1、cox2、nad2和nad5基因具有不完整的终止密码子(T)。美凤蝶富含A和T的区域包含一些与其他鳞翅目线粒体基因组中发现的保守结构相似的结构,包括一个结合了基序ATAGA的结构、一个19个碱基对的聚(T)延伸、一个微卫星(AT)n元件以及trnM上游的一个9个碱基对的聚(A)。美凤蝶线粒体基因组包含一个重复的36个碱基对的重复元件,它由一个26个碱基对的核心序列和两侧10个碱基对的完全反向重复组成。