Mirzamohammad Elham, Alirezalu Abolfazl, Alirezalu Kazem, Norozi Asadaolah, Ansari Afsaneh
Department of Biology Faculty of Sciences Urmia University Urmia Iran.
Department of Horticultural Sciences Faculty of Agriculture Urmia University Urmia Iran.
Food Sci Nutr. 2021 Oct 25;9(12):6873-6881. doi: 10.1002/fsn3.2643. eCollection 2021 Dec.
Recently, due to the valuable and high level of phytochemical compounds such as cannabinoids and other secondary metabolites, the cultivation of has increased in the world. The current study was conducted to evaluate the potential role of exogenous salicylic acid (control, 0.01, 0.1, and 1 M) on enhanced production of pharmaceutically important phytochemicals. The sprayed aerial parts were evaluated based on phenolic (TPC) and flavonoids (TFC) contents, antioxidant capacity (by FRAP and DPPH assay), photosynthetic pigments including chlorophyll a, b (Chl a and Chl b), total carotenoids (TCC), and cannabinoid compounds. Quantification of aerial parts metabolites was performed using gas chromatography. The results indicated that phytochemical compounds and antioxidant capacity in were influenced by various concentrations of salicylic acid (SA). The highest TPC, TFC, TCC, Chl a, Chl b, and antioxidant capacity were obtained in 1 M treatment, whereas the lowest of them were found in control plants. The major cannabinoids in the analyzed extracts were CBD (19.91%-37.81%), followed by Δ-THC (10.04%-22.84%), and CBL (nd-14.78%). The highest CBD (37.81%) and Δ-THC (22.84%) were obtained in 1 M of SA. These results suggest that the elicitor SA (especially 1 M) was able to improve antioxidant capacity, phytochemicals, and cannabinoid compounds.
最近，由于大麻素和其他次生代谢产物等植物化学化合物具有重要价值且含量高，其种植在全球范围内有所增加。本研究旨在评估外源水杨酸（对照、0.01、0.1和1 M）对增强具有重要药用价值的植物化学物质产量的潜在作用。根据酚类（TPC）和黄酮类（TFC）含量、抗氧化能力（通过FRAP和DPPH测定）、光合色素（包括叶绿素a、b（Chl a和Chl b）、总类胡萝卜素（TCC））以及大麻素化合物对喷洒的地上部分进行评估。使用气相色谱法对地上部分代谢产物进行定量分析。结果表明，大麻中的植物化学化合物和抗氧化能力受不同浓度水杨酸（SA）的影响。在1 M处理中获得了最高的TPC、TFC、TCC、Chl a、Chl b和抗氧化能力，而在对照植株中则最低。分析提取物中的主要大麻素为CBD（19.91%-37.81%），其次是Δ-THC（10.04%-22.84%）和CBL（未检出-14.78%）。在1 M的SA处理中获得了最高的CBD（37.81%）和Δ-THC（22.84%）。这些结果表明，诱导剂SA（尤其是1 M）能够提高抗氧化能力、植物化学物质和大麻素化合物的含量。
